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merck millipore,默克密理博,FCCH100181,FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kit (U20S)
产品名称：FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kit (U20S)
产品型号：FCCH100181
This FlowCellect GFP-LC3 Reporter Autophagy Assay Kit provide a quantitative solution for studying autophagy & measuring potency of autophagy inducers using flow cytometry.
merck millipore,默克密理博,FCCH100181,FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kit (U20S)
- 产品介绍
描述 产品目录编号 FCCH100181 商名 - FlowCellect
描述 FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kit (U20S) 概述 Millipore’s FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kits provide a quantitative solution for studying autophagy and measuring potency of autophagy inducers using flow cytometry. This kit has four unique features:
1.) The use of a proprietary selective permeabilization solution discriminates between cytosolic LC3 from autophagic LC3 by extracting the soluble cytosolic proteins, while protecting LC3 which has been sequestered into the autophagosome;
2.) The use of a monomeric GFP is used as a reporter to facilitate the translocation of the fusion protein, as other forms of GFP form dimers and aggregate when over-expressed in the cells, thus making it difficult to be extracted from cytoplasm and impossible to measure translocation by flow cytometry;
3.) Since autophagy is a constitutive cellular degradation process, the use of an autophagy detection reagent (Autophagy Reagent A) will prevent the lysosomal degradation of LC3, allowing its quantification by flow cytometry.
4.) The monomeric GFP used in our fusion protein to LC3 is attached on the 5' end (N-terminal fusion), protecting the GFP from Atg4 cleavage, thus, allowing its visualization within the autophagosomes.
A host cell line of U20S (human osteosarcoma) is offered to assist in your autophagy research. The CHO reporter cell line is ideal for flow cytometry applications, while U20S is suitable for both imaging and flow cytometry.Alternate Names - FlowCellect
背景信息 Autophagy is an intracellular catabolic pathway that causes cellular protein and organelle turnover, and it is associated in diverse diseases including Alzheimer's disease, aging, cancers, and Crohn's disease. It is a tightly regulated process that plays a normal part in cell growth, development, and cellular homeostasis. Autophagy functions as a housekeeping mechanism by disposing of aging and/or dysfunctional proteins and organelles by sequestering and priming for lysosomal degradation. Increasing evidence suggests that not just apoptosis, but autophagy can contribute to cell death and greatly influence general cell health. Malfunctions of autophagy can influence longevity and productivity of cells to function at full capacity.
During autophagy, LC3 protein is translocated from the cytoplasm to the autophagosome where it is targeted to the lysosome for degradation. The process of autophagy can be categorized into four distinct stages:
1) Induction and LC3 Translocation: The process is initiated by external/internal stimuli (e.g. nutrient
depletion);
2) Autophagosome formation: Unwanted cytosolic proteins and aging organelles are sequestered by a double membrane vesicle, i.e.—”autophagosome”. Formation of this vesicle is coordinated by complexes of Atg proteins (Atg5 and Atg12) that are conjugated, enabling the recruitment of LC3;
3) Lysosomal docking and fusion: LC3 protein regulates traffic between autophagosome to lysosome. (LC3-I is cytoplasmic; LC3-II is lipidated and sequestered into autophagosomal membrane);
4) Degradation: Fusion with the lysosome and subsequent breakdown of the autophagic vesicle and its contents.产品信息 部件 - 4. 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL
- 1. U20S cell background for FCCH100181 (Part No. CF200096) One vial containing 5 million cells
- 2. Autophagy reagent A: (Part No. CS208212) One vial (lyophilized)
- 3. Autophagy reagent B: (Part No. CS208215) One vial containing 1 mL
检测方法 Fluorescent 格式 Cell Line 应用 应用 This FlowCellect GFP-LC3 Reporter Autophagy Assay Kit provide a quantitative solution for studying autophagy & measuring potency of autophagy inducers using flow cytometry. 主要应用 - Flow Cytometry
生物信息 品种反应性 Human 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 This is a dual storage kit. All reagents must be stored at 2 - 8ºC, and the cell line must be stored at either -80°C (short term) or in liquid nitrogen (long term). 包装信息 数量 100 Tests
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