merck millipore,默克密理博,07-870,Anti-phospho-PKCβ I&II (Thr500) Antibody

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merck millipore,默克密理博,07-870,Anti-phospho-PKCβ I&II (Thr500) Antibody
产品名称：Anti-phospho-PKCβ I&II (Thr500) Antibody
产品型号：07-870
Detect phospho-PKCβ I&II (Thr500) using this Anti-phospho-PKCβ I&II (Thr500) Antibody validated for use in WB.

merck millipore,默克密理博,07-870,Anti-phospho-PKCβ I&II (Thr500) Antibody

产品介绍

merck millipore,默克密理博,07-870,Anti-phospho-PKCβ I&II (Thr500) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-870
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-PKCβ I&II (Thr500) Antibody
Alternate Names	PRKCB1, PRKCB2, PKCB, PKC beta, protein kinase C, beta 1&2 PRKCB1 PRKCB2 PKCB PKC beta protein kinase C

产品信息
格式	Affinity Purified
演示	100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.

应用
应用	Detect phospho-PKCβ I&II (Thr500) using this Anti-phospho-PKCβ I&II (Thr500) Antibody validated for use in WB.
主要应用	Western Blotting
应用说明	Immunoblot Analysis: A 1:1000 dilution of this lot detected PKCβ I&II in RIPA lysates from PMA treated K562 cells.

生物信息
免疫原品种	Peptide corresponding to amino acid region encompassing the human phospho-PKCβ I&II (Thr500)
宿主	Rabbit
特异性	Recognizes phosphorylated PKCβ I&II (Thr500). Minor cross reaction can occur with PKCα (Thr497), PKCγ (Thr514), and PKCε (Thr566) dependent upon cell type and treatment.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Predicted to cross-react with mouse and rat based sequence homology, but have not been tested.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_212535.1 NM_002738.5
Entrez基因汇总	Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
基因符号	PKC-beta PRKCB1 PRKCB PRKCB2 MGC41878 PKCB PKC-B
修改	Phosphorylation
纯化方法	Affinity Purfied
UniProt编号	P05771
UniProt汇总	FUNCTION: SwissProt: P05771 # This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May be considered as a novel component of the NF-kappa-B signaling axis responsible for the survival and activation of B-cells after BCR cross-linking (By similarity). COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity). SIZE: 671 amino acids; 76869 Da SUBUNIT: Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity). PTM: Phosphorylation on Thr-500 of isoform beta-I, within the activation loop, renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly, isoform beta-II is autophosphorylated on 'Thr-640' and 'Ser-659', subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i.e. in the N-terminal and hinge regions have no effect on PKC activity (By similarity). SIMILARITY: SwissProt: P05771 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.

产品使用声明
质量保证	Routinely evaluated by immunoblot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.