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merck millipore,默克密理博,05-1104,Anti-Insulin Receptor (β-Subunit) Antibody, clone CT-3
产品名称：Anti-Insulin Receptor (β-Subunit) Antibody, clone CT-3
产品型号：05-1104
Anti-Insulin Receptor (β-Subunit) Antibody, clone CT-3 detects level of Insulin Receptor (β-Subunit) & has been published & validated for use in ELISA, EA, WB, IH(P), IH.
merck millipore,默克密理博,05-1104,Anti-Insulin Receptor (β-Subunit) Antibody, clone CT-3
- 产品介绍
Replacement Information Replacement Information 05-1104 is a recommended replacement for MAB1139 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, Mk, M IHC, ELISA, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1104 Replaces MAB1139 描述 Anti-Insulin Receptor (β-Subunit) Antibody, clone CT-3 背景信息 The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin. 产品信息 格式 Purified 控制 - NIH/3T3 cell lysate
演示 Purified mouse monoclonal IgG1 in buffer containing 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. 应用 应用 Anti-Insulin Receptor (β-Subunit) Antibody, clone CT-3 detects level of Insulin Receptor (β-Subunit) & has been published & validated for use in ELISA, EA, WB, IH(P), IH. 主要应用 - Immunohistochemistry
- ELISA
- Western Blotting
应用说明 Immunohistochemistry (frozen and formalin/paraffin):
2-4ug/mL of a previous lot of this antibody was used in immunohistochemistry. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min.
Optimal working dilutions must be determined by end user.
ELISA: A previous lot of this antibody showed to be suitable as a capture antibody for ELISA.
Kinase Assay: A previous lot of this antibody was used for antibody-mediated capture on microplates.
Western Blot: A previous lot of this antibody was used at 1 ug/mL, for 2 hrs at room temperature.
生物信息 免疫原品种 Recombinant-fragment including the C-terminal 100 amino acids of human insulin receptor, β subunit. 表位 100 C-Terminal amino acids of human insulin receptor, β-subunit. 克隆 CT-3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes a protein of 95 kDa, identified as the β-subunit of insulin receptor (IR). It is directed against the C-terminal region of β-subunit and shows no cross-reaction with IGF-receptors. 同种型 IgG1 品种反应性 RatHumanMonkeyMouse Species Reactivity Note Human, Monkey, Mouse and Rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000208.2
Entrez基因汇总 After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
基因符号 - IR
- INSR
- CD220
- HHF5
纯化方法 Protein G purfied UniProt编号 - P06213
UniProt汇总 FUNCTION: This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. Mediates the metabolic functions of insulin. Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). Can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
ENZYME REGULATION: Autophosphorylation activates the kinase activity.
SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. Interacts with SORBS1 but dissociates from it following insulin stimulation. Binds SH2B2. Interacts with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. The sequences surrounding the phosphorylated NPXY motif contribute differentially to either IRS1 or SHC1 recognition. Interacts with the SH2 domains of the 85 kDa regulatory subunit of PI3K (PIK3R1) in vitro, when autophosphorylated on tyrosine residues. Interacts with SOCS7.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative产品使用声明 质量保证 Routinely evaluated by Western Blot on NIH/3T3 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected Insulin Receptor, beta subunit on 10 μg of NIH/3T3 lysates.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C in undiluted aliquots from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,SF-1701,GPR124; Human, Cyanine 5 RNA Detection Probe,SmartFlare
下一件merck millipore产品：merck millipore,默克密理博,71397,Rosetta™ 2(DE3) Competent Cells - Novagen
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