merck millipore,默克密理博,FCCH100140,FlowCellect™ Human CD8 T Cell FAS Kit

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,FCCH100140,FlowCellect™ Human CD8 T Cell FAS Kit
产品名称：FlowCellect™ Human CD8 T Cell FAS Kit
产品型号：FCCH100140
The FlowCellect CD8 T Cell FAS Kit for Flow Cytometry allows for the identification & counts of CD3 T cells & CD8 cytotoxicity T cells & monitoring FAS expression levels.

merck millipore,默克密理博,FCCH100140,FlowCellect™ Human CD8 T Cell FAS Kit

产品介绍

merck millipore,默克密理博,FCCH100140,FlowCellect™ Human CD8 T Cell FAS Kit

描述
产品目录编号	FCCH100140
商名	FlowCellect
描述	FlowCellect™ Human CD8 T Cell FAS Kit
概述	The FlowCellect CD8 T Cell FAS kit allows for the identification and counts of CD3 T cells and CD8 cytotoxicity T cells and monitoring FAS expression levels by enabeling the detection and quantitation of CD95 (Apo1, FAS) marker on either whole blood or PBMC using simplified no-wash assays. The kit can be utilized on any flow cytometry system equipped with blue laser. The performance of these assays on the guava easyCyte 8HT platform along with the Incyte software allows for cell count information on CD8, CD3 T cells and count and % of populations expressing CD95/FAS. Furthermore, easy identification of activated T cells can be performed using heat map features or measurement of the kinetics of activation using the dose response features. The simplified identification of activated samples can be of great utility in drug development, kinetic studies and understanding mechanism of compound action and disease.
Alternate Names	Flow Cellect
背景信息	FAS expression and apoptosis has been shown to play a significant role in T-cell selection in the thymus for the acquisition of self-tolerance, and in T-cell mediated cytotoxicity and in downregulation of the immune response. It has also a role in the clonal deletion of activated T-cells in order to maintain T cell homeostasis, down-regulation of inflammatory T cells and the regulation of activated B cells. The presence or absence of FAS and/or FasL on cells of the immune system and their interaction with FAS and FasL on cells of other tissues plays a major factor in immune system homeostasis. It has been proposed that AICD serves a role in limiting the expansion of an immune response by eliminating lymphocytes that are no longer necessary at the end of an infection. Recent studies have also indicated that in addition to a critical role in receptor mediated apoptosis, FAS has a growth-promoting role during tumorigenesis.

产品信息
部件	5. Guava 1X Lysing Solution (Part No.4700-0082) One bottle containing 40 mL of 1X Lysing Solution 1. CD8 FITC/ CD3PECY5 Cocktail (Part No.4700-1405 One vial containing 500 µL of Antibody Cocktail. 2. Anti-Human CD95(FAS)-PE Antibody (Part No. 4700-1395) One vial containing 500 µL of labeled antibody. 3. Isotype control mouse IgG1-PE (Part No. 4700-1390) One vial containing 500 µL of labeled antibody. 4. 1X Assay Buffer BA ((Part No.4700-1360) One bottle containing 50 mL of 1x Assay Buffer.
检测方法	Fluorescent

产品信息

部件

5. Guava 1X Lysing Solution (Part No.4700-0082) One bottle containing 40 mL of 1X Lysing Solution
1. CD8 FITC/ CD3PECY5 Cocktail (Part No.4700-1405 One vial containing 500 µL of Antibody Cocktail.
2. Anti-Human CD95(FAS)-PE Antibody (Part No. 4700-1395) One vial containing 500 µL of labeled antibody.
3. Isotype control mouse IgG1-PE (Part No. 4700-1390) One vial containing 500 µL of labeled antibody.
4. 1X Assay Buffer BA ((Part No.4700-1360) One bottle containing 50 mL of 1x Assay Buffer.

检测方法

Fluorescent

应用
应用	The FlowCellect CD8 T Cell FAS Kit for Flow Cytometry allows for the identification & counts of CD3 T cells & CD8 cytotoxicity T cells & monitoring FAS expression levels.
主要应用	Flow Cytometry

生物信息
宿主	Mouse
品种反应性	Human
抗体类型	Monoclonal Antibody

产品使用声明
质量保证	Lot to lot in-process testing on all kit components
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.