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  • merck millipore,默克密理博,07-1016,Anti-phospho-Upf1 (Ser1127) Antibody

  • merck millipore,默克密理博,07-1016,Anti-phospho-Upf1 (Ser1127) Antibody

    产品名称：Anti-phospho-Upf1 (Ser1127) Antibody
    产品型号：07-1016
    Anti-phospho-Upf1 (Ser1127) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Upf1 also known as Regulator of nonsense transcripts 1 or NORF1 & has been validated in WB & IP.

    merck millipore,默克密理博,07-1016,Anti-phospho-Upf1 (Ser1127) Antibody

  • 产品介绍
  • merck millipore,默克密理博,07-1016,Anti-phospho-Upf1 (Ser1127) Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Ch, H, Xn, B, M, RWBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 07-1016
    描述 Anti-phospho-Upf1 (Ser1127) Antibody
    Alternate Names
    • hUpf1
    • Regulator of nonsense transcripts 1
    • ATP-dependent helicase RENT1
    • Nonsense mRNA reducing factor 1
    • NORF1
    • Up-frameshift suppressor 1 homolog
    背景信息 Upf1 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. In mammalian cells NMD generally requires that translation terminates upstream of a post-splicing exon junction complex (EJC). The subsequent binding of Upf1 to the EJC triggers Upf1 phosphorylation. Data suggests that phospho-Upf1 interacts directly with eIF3 and inhibits the eIF3-dependent conversion of 40S ribosome complex to translationally competent 80S ribosome initiation complex to repress continued translation initiation.
    产品信息
    格式 Affinity Purified
    控制
    • Lamda phosphatase treated and untreated Jurkat RIPA lysates
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Anti-phospho-Upf1 (Ser1127) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Upf1 also known as Regulator of nonsense transcripts 1 or NORF1 & has been validated in WB & IP.
    主要应用
    • Western Blotting
    应用说明 Western Blot Analysis: A representative lot detected Upf1 in Xenopus egg extract.
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to Upf1 phosphorylated at Ser1127.
    表位 Phosphorylated Ser1127
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes Upf1 phosphorylated at Ser1127.
    品种反应性 ChickenHumanXenopusBovineMouseRat
    Species Reactivity Note Demonstrated to react with Xenopus and Human. Predicted on Bovine, Mouse, Rat, Chicken, Yeast, and Zebra fish based on 100% sequence homology.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_002902
    Entrez基因汇总 This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq].
    基因符号
    • RENT1
    • UPF1
    修改
    • Phosphorylation
    纯化方法 Affinity purified
    UniProt编号
    • Q92900
    UniProt汇总 FUNCTION: Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.

    SUBUNIT STRUCTURE: Found in a complex with UPF1, UPF2, UPF3A and UPF3B. Found in a complex with PARN. Found in a post-splicing complex with SMG1, NXF1, RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1. Found in a mRNA decay complex with EXOSC2, EXOSC4, EXOSC10, PARN, XRN1, DCP2, UPF1, UPF2 and UPF3B. Interacts with EST1A, UPF2 and SLBP. Component of the SMG1C complex, at least composed of SMG1, SMG8 and SMG9. The SMG1C complex is then recruited on premature translation termination codons (PTCs) to form the ribosome:SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9. Interacts (when hyperphosphorylated) with PNRC2. Ref.16 Ref.8 Ref.11 Ref.15 Ref.24

    SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › P-body. Note: Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.

    TISSUE SPECIFICTY: Ubiquitous.

    DOMAIN: The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.

    PTM: Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.

    SEQUENCE SIMILARITIES: Belongs to the DNA2/NAM7 helicase family.

    Contains 1 C2H2-type zinc finger.

    SEQUENCE CAUTION: The sequence BAA19664.2 differs from that shown. Reason: Erroneous initiation.
    产品使用声明
    质量保证 Evaluated by Immunoprecitipation in lamda phosphatase treated and untreated Jurkat RIPA lysates.

    Immunoprecitipation Analysis: 20 µg of Anti-phospho-Upf1 (Ser1127) immunoprecipitated phospho-Upf1 (Ser1127) from lamda phosphatase treated and untreated Jurkat RIPA lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,07-1016,Anti-phospho-Upf1 (Ser1127) Antibody

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