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merck millipore,默克密理博,ECM550,QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric
产品名称：QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric
产品型号：ECM550
The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
merck millipore,默克密理博,ECM550,QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric
- 产品介绍
重要规格表
检测方法 Chromogenic 描述 产品目录编号 ECM550 品牌系列 Chemicon®
商名 - Chemicon
- QCM
描述 QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric 概述 Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay!
Introduction
Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade.
The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrix™, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor. We examined the kit's performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3).
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Test Principle:
The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain an 8 μm pore size polycarbonate membrane, over which a thin layer of ECMatrixTM is dried. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane.
Application:
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.产品信息 部件 - Forceps: (Part No. 10203) One each.
- Invasion Chamber: (Part No. 70019) One 24-well plate with 12 ECMatrixTM layered cell culture inserts.
- Cell Stain: (Part No. 20294) One 10 mL bottle.
- Cotton Swabs: (Part No. 10202) 24 each.
检测方法 Chromogenic 应用 应用 The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. 应用说明 Detection of migrating cells is via crystal violet stain. 生物信息 品种反应性 Vertebrates 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store kit materials at 2-8°C for up to 6 months. Do not freeze. 包装信息 数量 1 plate 包装 12 wells
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