merck millipore,默克密理博,ECM300,QuantiMatrix Human Fibronectin ELISA

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merck millipore,默克密理博,ECM300,QuantiMatrix Human Fibronectin ELISA
产品名称：QuantiMatrix Human Fibronectin ELISA
产品型号：ECM300
The QuantiMatrix ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants & purified protein samples.

merck millipore,默克密理博,ECM300,QuantiMatrix Human Fibronectin ELISA

产品介绍

merck millipore,默克密理博,ECM300,QuantiMatrix Human Fibronectin ELISA

重要规格表

可用的分析物	品种反应性	主要应用	检测方法
Fibronectin	H	ELISA	Chromogenic

描述
产品目录编号	ECM300
品牌系列	Chemicon®
商名	Chemicon Quantimatrix
描述	QuantiMatrix Human Fibronectin ELISA
概述	The QuantiMatrix™ ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants and purified protein samples. The assay is in the format of a competitive inhibition ELISA. Human fibronectin is pre-coated on the wells with the exception of row H, which serves as a reference. Standards and samples are diluted and pre-incubated with polyclonal rabbit antibody to human fibronectin. The polyclonal antibody binds to fibronectin in the standard dilutions, and in the sample, if present. The mixture is then transferred to the human fibronectin-coated plate. Free rabbit anti-human fibronectin binds to the fibronectin on the plate. Goat anti-rabbit IgG HRP conjugate reacts with bound rabbit anti-fibronectin. When HRP substrate is added, a blue color develops. The reaction is stopped by the addition of an acid, changing the color to yellow. This color is quantitated using an ELISA reader; the intensity of the color is inversely proportional to the amount of fibronectin in the original sample. A standard curve is constructed and sample values are interpolated.
背景信息	The determination of levels of extracellular matrix molecules is becoming more important to understanding alterations in matrix function during development and tumorigenesis. Questions of matrix gene regulation and function require a sensitive means of determining the levels of protein expression by cells. The QuantiMatrix™ ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants and purified protein samples. The assay is in the format of a competitive inhibition ELISA. Human fibronectin is pre-coated on the wells with the exception of row H, which serves as a reference. Standards and samples are diluted and pre-incubated with polyclonal rabbit antibody to human fibronectin. The polyclonal antibody binds to fibronectin in the standard dilutions, and in the sample, if present. The mixture is then transferred to the human fibronectin-coated plate. Free rabbit anti-human fibronectin binds to the fibronectin on the plate. Goat anti-rabbit IgG HRP conjugate reacts with bound rabbit anti-fibronectin. When HRP substrate is added, a blue color develops. The reaction is stopped by the addition of an acid, changing the color to yellow. This color is quantitated using an ELISA reader; the intensity of the color is inversely proportional to the amount of fibronectin in the original sample. A standard curve is constructed and sample values are interpolated.

产品信息
部件	900 mL of deionized water as described in step 1 of protocol on page 2. QuantiMatrixTM Fibronectin Coated Strips (Part No. 60103) - 1 x 96-well strip plate Human Fibronectin Standard (Part No. 60109) - 1 lyophilized bottle; reconstitute according to the protocol on page 2 Sample Diluent (Part No. 60095) - 1 x 15 mL bottle of 1% BSA in PBS + 0.01% Thimerosal Goat anti-Rabbit IgG-HRP Conjugate (QM2) (Part No. 60110) - 1 bottle; ready to use Rabbit anti-Human Fibronectin (Part No. 60111) - 1 bottle pre-diluted polyclonal antibody; ready to use TMB/E Substrate (Part No. 60096) - 1 bottle; ready to use Stop Solution (Part No. 60193) - 1 bottle; (0.5M HCl solution) Wash Buffer Concentrate (Part No. 60245) - 1 x 100 mL bottle; provides final concentrations of 0.05% Tween-20 and 0.01% Thimerosal when added to
检测方法	Chromogenic

产品信息

部件

900 mL of deionized water as described in step 1 of protocol on page 2.
QuantiMatrixTM Fibronectin Coated Strips (Part No. 60103) - 1 x 96-well strip plate
Human Fibronectin Standard (Part No. 60109) - 1 lyophilized bottle; reconstitute according to the protocol on page 2
Sample Diluent (Part No. 60095) - 1 x 15 mL bottle of 1% BSA in PBS + 0.01% Thimerosal
Goat anti-Rabbit IgG-HRP Conjugate (QM2) (Part No. 60110) - 1 bottle; ready to use
Rabbit anti-Human Fibronectin (Part No. 60111) - 1 bottle pre-diluted polyclonal antibody; ready to use
TMB/E Substrate (Part No. 60096) - 1 bottle; ready to use
Stop Solution (Part No. 60193) - 1 bottle; (0.5M HCl solution)
Wash Buffer Concentrate (Part No. 60245) - 1 x 100 mL bottle; provides final concentrations of 0.05% Tween-20 and 0.01% Thimerosal when added to

检测方法

Chromogenic

应用
应用	The QuantiMatrix ELISA kit for the determination of soluble human fibronectin provides sensitivity down to 10-20 ng/mL as determined from culture supernatants & purified protein samples.
主要应用	ELISA

生物信息
品种反应性	Human
可用的分析物	Fibronectin
Entrez基因编号	NM_212482.1 NM_002026.2 NM_054034.2 NM_212474.1 NM_212475.1 NM_212476.1 NM_212478.1
Entrez基因汇总	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
基因符号	FINC FN1 DKFZp686O13149 DKFZp686H0342 DKFZp686I1370 MSF FN CIG DKFZp686F10164 LETS
UniProt编号	P02751
UniProt汇总	FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity). SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. PTM: Sulfated. SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store the kit and all components at 2-8°C. PRECAUTIONS: Human fibronectin was purified from human plasma that tested negative for HBsAg and HIV. However, all materials should be handled carefully and in accordance with good laboratory practices. The instructions provided have been designed to optimize performance. Deviations from the suggested procedure may result in sub-optimal performance of the kit and failure to produce accurate data. Do not mix or substitute reagents from different kit lots.

储存和货运信息

存储条件

Store the kit and all components at 2-8°C.

PRECAUTIONS:

Human fibronectin was purified from human plasma that tested negative for HBsAg and HIV. However, all materials should be handled carefully and in accordance with good laboratory practices.

The instructions provided have been designed to optimize performance. Deviations from the suggested procedure may result in sub-optimal performance of the kit and failure to produce accurate data.

Do not mix or substitute reagents from different kit lots.