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重要规格表
主要应用 CULT, Cell Attachment 描述 产品目录编号 CC130 商名 - PluriSTEM
描述 PluriSTEM-XF™ Recombinant Vitronectin Alternate Names - V75
- S-protein
- Serum-spreading factor
背景信息 PluriSTEM™ Recombinant Vitronectin is an effective xeno-free alternative to matrigel™ and has been validated to successully culture human ES and iPS cells using PluriSTEM™ Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.
PluriSTEM™ Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.产品信息 演示 Product is sterile-filtered in a 20 mM pH 8.0 Tris-HCl buffer, with a proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and glycerol. 应用 应用 Human pluripotent stem cell culture. 主要应用 - Cell Culture
- Cell Attachment
应用说明 Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions. 生物信息 浓缩 0.5 mg/mL 宿主 E. coli 纯度 Product is ≥ 95% pure by SDS-PAGE. 品种 Human 基因符号 - VTN
UniProt编号 - P04004
细胞系类型 - Embryonic Stem Cells
产品使用声明 质量保证 Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate). 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles. 包装信息 数量 500 µg
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