merck millipore,默克密理博,04-400,Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal

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merck millipore,默克密理博,04-400,Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal
产品名称：Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal
产品型号：04-400
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-PI3 Kinase Antibody, p110β, Rabbit is vali More>>

merck millipore,默克密理博,04-400,Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-400,Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB, FC	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-400
品牌系列	Upstate
商名	Upstate
描述	Anti-PI3 Kinase Antibody, p110β, rabbit monoclonal
Alternate Names	phosphoinositide-3-kinase, catalytic, beta polypeptide PI3-kinase p110 subunit beta PtdIns-3-kinase p110 phosphatidylinositol 3-kinase, catalytic, beta polypeptide catalytic beta polypeptide
背景信息	PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

产品信息
格式	Purified
控制	Jurkat cell lysate
演示	Purified rabbit monoclonal IgG in buffer containing 0.03% BSA, 30 mM Tris-Glycine (pH 7.4), 0.09 M NaCl, 0.006% sodium azide as a preservative in a solution of 40% glycerol

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-PI3 Kinase Antibody, p110β, Rabbit is validated for use in FC, IP, WB for the detection of PI3 Kinase.
主要应用	Western Blotting Flow Cytometry
应用说明	Immunoprecipitation: A 1:50 dilution of a previous lot of this antibody immunoprecipitated PI3 Kinase, p110β using protein G:agarose beads. Flow Cytometry: A 1:40 of a previous lot of this antibody stained cells for intracellular staining for flow Cytometry analysis.

生物信息
免疫原品种	KLH-conjugated synthetic peptide corresponding to the N-terminal region of human PI3 Kinase, p110β
表位	N-terminus
克隆	Y384
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes a region in the N-terminus domain of human PI3 Kinase, p110β. Non specific band detected at MW 55 kDa.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react with human. Reactivity with other species has not been confirmed.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_006219.1
Entrez基因汇总	Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]
基因符号	DKFZp779K1237 PIK3CB PI3Kbeta MGC133043 PIK3C1
纯化方法	Protein A purfied
UniProt编号	P42338
UniProt汇总	FUNCTION: SwissProt: P42338 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. SIZE: 1070 amino acids; 122762 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. TISSUE SPECIFICITY: Expressed ubiquitously. SIMILARITY: SwissProt: P42338 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on jurkat cell lysate. Western Blot Analysis: A 1:500 dilution of this lot detected endogenous PI3 Kinase, p110β in lysate from Jurkat cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage