merck millipore,默克密理博,ABN22,Anti-UCH-L1 Antibody

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,ABN22,Anti-UCH-L1 Antibody
产品名称：Anti-UCH-L1 Antibody
产品型号：ABN22
This Anti-UCH-L1 Antibody is validated for use in WB for the detection of UCH-L1.

merck millipore,默克密理博,ABN22,Anti-UCH-L1 Antibody

产品介绍

merck millipore,默克密理博,ABN22,Anti-UCH-L1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB	Ch	Purified	Polyclonal Antibody

描述
产品目录编号	ABN22
描述	Anti-UCH-L1 Antibody
Alternate Names	Ubiquitin thioesterase L1 Ubiquitin carboxyl-terminal hydrolase isozyme L1 UCH-L1 Neuron cytoplasmic protein 9.5 PGP 9.5 PGP9.5
背景信息	Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-termial glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and thier tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5.

产品信息
格式	Purified
控制	PC12 cell lysate
演示	Purified chicken polyclonal in PBS containing 0.05% sodium azide and 10% glycerol.

应用
应用	This Anti-UCH-L1 Antibody is validated for use in WB for the detection of UCH-L1.
主要应用	Western Blotting

生物信息
免疫原品种	Histidine-tagged recombinant protein corresponding to human UCH-L1.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Chicken
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with Human, Mouse, and Rat.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_004172
Entrez基因汇总	The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
基因符号	UCHL1
纯化方法	IgY purified
UniProt编号	P09936
UniProt汇总	FUNCTION: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. CATALYTIC ACTIVITY: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). SUBUNIT STRUCTURE: Monomer. Homodimer. Interacts with SNCA By similarity. Interacts with COPS5. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. PTM: O-glycosylated (By similarity). INVOLVEMENT IN DISEASE: Defects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia. MISCELLANEOUS: Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles. In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8. SEQUENCE SIIMILARITIES: Belongs to the peptidase C12 family. Caution Ref.8 reports the association of mutation Ile93Met with Parkinson disease. However, according to Ref.26 this association is uncertain and UCHL1 is not a susceptibility gene for Parkinson disease. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=122 nM for Ub-AMC KM=1.20 µM for ubiquitin ethyl ester Vmax=0.47 µmol/min/mg enzyme toward Ub-AMC Vmax=25 µmol/min/mg enzyme toward ubiquitin ethyl ester SEQUENCE CAUTION: The sequence CAA28443.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

产品使用声明
质量保证	Evaluated by Western Blot in PC12 cell lysate. Western Blot Analysis: 0.2 µg/mL of this antibody detected UCH-L1 on 10 µg of PC12 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.