merck millipore,默克密理博,QIA120,Calpain Activity Assay Kit, Fluorogenic

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merck millipore,默克密理博,QIA120,Calpain Activity Assay Kit, Fluorogenic
产品名称：Calpain Activity Assay Kit, Fluorogenic
产品型号：QIA120

merck millipore,默克密理博,QIA120,Calpain Activity Assay Kit, Fluorogenic

产品介绍

merck millipore,默克密理博,QIA120,Calpain Activity Assay Kit, Fluorogenic

重要规格表

检测方法
Fluorometric

描述
概述	Calpains are Ca²⁺-dependent proteinases, which are involved in normal cellular processes including cell proliferation, apoptosis, differentiation, and cell migration. Requires a fluorimeter or microplate reader capable of measuring fluorescence at wavelengths of Excitation max: ~360 nm and Emission max: ~460 nm.
产品目录编号	QIA120
品牌系列	Calbiochem®
应用数据	Typical standard curve generated by using dilutions of the AMC Standard as indicated in the Detailed Protocol.

参考
参考	Dainese, E., et al. 2002. J. Biol. Chem. 277, 40296. Glading, A., et al. 2000. J. Biol. Chem. 275, 23908. Ishikara, I., et al. 2000. Neurosci. Lett. 279, 97. Nakagawa, T. and Yuan, J. 2000. J. Cell Biol. 150, 887. Pariat, M., et al. 2000. Biochem. J. 345, 129. Pink, J.J., et al. 2000.Exp. Cell Res. 255, 144. Wang, K.K. 2000. Trends Neurosci. 23, 20. Reddy, R.K., et al. 1999. J. Biol. Chem. 274, 28476. Leist, M., et al. 1998. Mol. Pharmacol. 54, 789. Melloni, E., et al. 1998. J. Biol. Chem. 273, 12827. Villa, P.G., et al. 1998. J. Cell Sci. 111, 713. Wood, D.E., et al. 1998. Oncogene 17, 1069. Kubbutat, M.H. and Vousden, K.H. 1997. Mol. Cell Biol. 17, 460. Molinari, M. and Carafoli, E. 1997. J. Membr. Biol. 156, 1. Sorimachi, H., et al. 1997. Biochem. J. 328, 721. Squier, M.K. and Cohen, J.J. 1997. J. Immunol. 158, 3690. Aoki, K., et al. 1986. FEBS Lett. 205, 313. Ohno, S., et al. 1986. Nucleic Acid Res. 14, 5559.

参考

Dainese, E., et al. 2002. J. Biol. Chem. 277, 40296.
Glading, A., et al. 2000. J. Biol. Chem. 275, 23908.
Ishikara, I., et al. 2000. Neurosci. Lett. 279, 97.
Nakagawa, T. and Yuan, J. 2000. J. Cell Biol. 150, 887.
Pariat, M., et al. 2000. Biochem. J. 345, 129.
Pink, J.J., et al. 2000.Exp. Cell Res. 255, 144.
Wang, K.K. 2000. Trends Neurosci. 23, 20.
Reddy, R.K., et al. 1999. J. Biol. Chem. 274, 28476.
Leist, M., et al. 1998. Mol. Pharmacol. 54, 789.
Melloni, E., et al. 1998. J. Biol. Chem. 273, 12827.
Villa, P.G., et al. 1998. J. Cell Sci. 111, 713.
Wood, D.E., et al. 1998. Oncogene 17, 1069.
Kubbutat, M.H. and Vousden, K.H. 1997. Mol. Cell Biol. 17, 460.
Molinari, M. and Carafoli, E. 1997. J. Membr. Biol. 156, 1.
Sorimachi, H., et al. 1997. Biochem. J. 328, 721.
Squier, M.K. and Cohen, J.J. 1997. J. Immunol. 158, 3690.
Aoki, K., et al. 1986. FEBS Lett. 205, 313.
Ohno, S., et al. 1986. Nucleic Acid Res. 14, 5559.

产品信息
检测方法	Fluorometric
形式	96 Tests
格式	96-well plate
试剂盒包含	Calibration Standard, Positive Control Calpain 1, Substrate, Activation Buffer, Inhibition Buffer, Reduction Agent, Assay Buffer, Cell Lysis Buffer, 96-Well Plate, Plate Sealer, and a user protocol.
正控制	Calpain 1

生物信息
化验时间	1.5 h
样品类型	Cell lysates, plasma, serum

储存和货运信息
货运代码	Multiple Storage Conditions
毒性	Multiple Toxicity Values, refer to MSDS
有害材料注意事项：	Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存	Multiple storage requirements
存储条件	Upon arrival, store the Control Human Calpain 1 at -70°C and the remaining components at -20°C. With the exception of the Control Human Calpain 1, all components, once opened, can be stored at 4°C for up to 1 month.
避光	Protect from light
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No

补充信息
试剂盒包含	Calibration Standard, Positive Control Calpain 1, Substrate, Activation Buffer, Inhibition Buffer, Reduction Agent, Assay Buffer, Cell Lysis Buffer, 96-Well Plate, Plate Sealer, and a user protocol.