- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, FC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 05-253 品牌系列 Upstate
商名 - Upstate
描述 Anti-ZAP-70 Antibody, clone 2F3.2 概述 Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals. 背景信息 Zeta-chain-associated protein kinase 70. A member of the protein tyrosine kinase family, ZAP-70 is normally expressed in T cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 is expressed in T cells and tumors of T-cell lineage. A high level of ZAP-70 expression appears restricted to T-cell proliferative diseases and a subgroup of chronic lymphocytic leukemia (CLL). The ZAP-70 gene is in chromosome 2q12. 产品信息 格式 Purified 控制 - Positive Control Included: Jurkat cell lysate (12-303)
演示 Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative. 应用 应用 This Anti-ZAP-70 Antibody, clone 2F3.2 is validated for use in FC, IH, IP, WB for the detection of ZAP-70. 主要应用 - Western Blotting
- Flow Cytometry
- Immunohistochemistry
应用说明 For testing in IHC, please see the following link (PDF): 生物信息 免疫原品种 GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254 克隆 2F3.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes human ZAP-70, does not recognize murine ZAP-70. 同种型 IgG2a 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001079
Entrez基因汇总 This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. 基因符号 - SRK
- ZAP70
- ZAP-70
- TZK
- STD
纯化方法 Protein G purfied UniProt编号 - P43403
UniProt汇总 FUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
SIZE: 619 amino acids; 69872 Da
SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.产品使用声明 质量保证 routinely evaluated on RIPA lysates prepared from Jurkat cells or Hut-78 cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 250 µg
上一件merck millipore产品：merck millipore,默克密理博,PC130,Anti-Rad51 (Ab-1) Rabbit pAb
下一件merck millipore产品：merck millipore,默克密理博,QIA120,Calpain Activity Assay Kit, Fluorogenic
相关产品
merck millipore,默克密理博,ELI-018-H,TNFα ELISPOT Antibody Pair, Human
merck millipore,默克密理博,528283,Polo-like Kinase Inhibitor II, BTO-1 - CAS 40647-02-7 - Calbiochem
merck millipore,默克密理博,69659,pLysS DNA - Novagen
merck millipore,默克密理博,AG30S-K,Human IgE Myeloma (1mg)
merck millipore,默克密理博,PC130,Anti-Rad51 (Ab-1) Rabbit pAb
merck millipore,默克密理博,QIA120,Calpain Activity Assay Kit, Fluorogenic
merck millipore,默克密理博,401489,IKK Inhibitor X - CAS 431898-65-6 - Calbiochem
merck millipore,默克密理博,539741,PTP1B Inhibitor - CAS 765317-72-4 - Calbiochem
merck millipore,默克密理博,MAB8044,Anti-Adenovirus 1,2,5,6 Antibody, clone 7/48-7a
merck millipore,默克密理博,EZGRA-88K,Human Ghrelin (active) ELISA