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merck millipore,默克密理博,CBL171,Anti-Actin Antibody, smooth muscle, clone ASM-1
产品名称：Anti-Actin Antibody, smooth muscle, clone ASM-1
产品型号：CBL171
Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.
merck millipore,默克密理博,CBL171,Anti-Actin Antibody, smooth muscle, clone ASM-1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B, Ch, Eq, H, M WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 CBL171 Replaces 04-1040 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Actin Antibody, smooth muscle, clone ASM-1 Alternate Names - Alpha-actin-2
背景信息 ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast. 产品信息 格式 Purified 控制 - Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
演示 Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.应用 应用 Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin. 表位 N-terminus 克隆 ASM-1 宿主 Mouse 特异性 This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa). 同种型 IgG2a 品种反应性 RatBovineChickenHorseHumanMouse Species Reactivity Note Human, mouse, and rat. Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001604
Entrez基因汇总 The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] 基因符号 - AAT6
- ACTA2
- ACTSA
纯化方法 Protein A purfied UniProt编号 - P62736
UniProt汇总 FUNCTION: SwissProt: P62736 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SIZE: 377 amino acids; 42009 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
SIMILARITY: SwissProt: P62736 ## Belongs to the actin family.
MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.产品使用声明 质量保证 Evaluated by Western Blot on HUVEC lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 50 µg
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