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重要规格表
检测方法 Fluorometric 描述 概述 A cell adhesion array intended for the determination of the relative attachment of adherent cell lines to 6 different ECM proteins-laminin, fibronectin, vitronectin, and collagen type I, type III, and type IV. 产品目录编号 CBA083 品牌系列 Calbiochem®
应用数据 
Approximately 30,000 cells were added to wells containing fibronectin, laminin I, collagen type IV, vitronectin, basement membrane complex, and BSA and incubated for 1.5 h at 37°C in a cell culture incubator in the presence of 6% CO2. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in a cell culture incubator in the presence of 6% CO2. Fluorescence was measured as outlined in the Detailed Protocol.
Approximately 30,000 cells were added to wells containing collagen I, III, or IV, and incubated for 1.5 h at 37°C in a cell culture incubator in the presence of 6% CO2. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in a cell culture incubator in the presence of 6% CO2. Fluorescence was measured as outlined in the Detailed Protocol.
Approximately 10,000 cells were added to wells coated with laminin I in the presence or absence of inhibitory antibody, anti-integrin b1 (Cat. No. CP26). The cells were incubated and labeled and fluorescence was measured as outlined in Figure 1.
参考 参考 Stupack, D.G. 2005. Cell Death and Differentiation 12, 1021.
Ekblom, P., et al. 2003.Matrix Biology 22, 35.
Li, S., et al. 2002. J. Cell Biol. 157, 1279.
Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2588.
Aumailley, M., et al. 1998. J. Anat. 193, 1.
Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104.
Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460.
Frisch, S.M. 1994. J.Cell Biol. 124, 701.
Meredith J.E. 1993. Mol. Biol. Cell 4, 953.产品信息 检测方法 Fluorometric 形式 96 Tests (14 samples x 6 ECM proteins and 12 negative controls) 格式 96-well plate 试剂盒包含 ECM Protein-Coated 96-Well Plate Strips, Calcein-AM Solution, D-PBS, and a user protocol. 安全信息 R相 R: 36/38
Irritating to eyes and skin.S相 S: 26
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.产品使用声明 预定用途 The Calbiochem® InnoCyte™ 96-Well Cell Adhesion Array is intended for the determination of the relative attachment of adherent cell lines to 6 different extracellular matrix (ECM) proteins: laminin, fibronectin, vitronectin, and collagens type I, type III, and type IV. BSA-coated wells serve as negative control. 储存和货运信息 货运代码 Blue Ice Only
毒性 Irritant
储存 Multiple storage requirements
存储条件 Upon arrival store the Calcein-AM at -20°C and the remaining components of the kit at 4°C. 无需冷冻 No
补充信息 试剂盒包含 ECM Protein-Coated 96-Well Plate Strips, Calcein-AM Solution, D-PBS, and a user protocol.
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