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merck millipore,默克密理博,04-1150,Anti-MMP-9 Antibody, clone EP1254, rabbit monoclonal
产品名称：Anti-MMP-9 Antibody, clone EP1254, rabbit monoclonal
产品型号：04-1150
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-MMP-9 Antibody, clone EP1254, Rabbit detects le More>>
merck millipore,默克密理博,04-1150,Anti-MMP-9 Antibody, clone EP1254, rabbit monoclonal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H FC, WB, ICC Rb Unpurified Monoclonal Antibody 描述 产品目录编号 04-1150 描述 Anti-MMP-9 Antibody, clone EP1254, rabbit monoclonal Alternate Names - type V collagenase
- 92 kDa gelatinase
- 92 kDa type IV collagenase
- 92kD type IV collagenase
- Gelatinase B
- macrophage gelatinase
- matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
- matrix metalloproteinase 9
- matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase)
- matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
背景信息 All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn2+-binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion.
MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas.产品信息 格式 Unpurified 控制 - U937, HL-60, and HT-1080 cell lysates
演示 Rabbit Monoclonal in buffer containing 50 mM Tris-glycine (pH 7.4), 0.15 M NaCl containing 40% glycerol, 0.01% sodium azide and 0.05% BSA. 应用 应用 Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-MMP-9 Antibody, clone EP1254, Rabbit detects level of MMP-9 & has been published & validated for use in IH(P), WB, IC, FC. 主要应用 - Flow Cytometry
- Western Blotting
- Immunocytochemistry
应用说明 Immunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected MMP-9 in human gastric adenocarcinoma tissue.
Immunocytochemistry Analysis: 1:250 - 500 dilution from a previous lot detected MMP-9 in HeLa cell lysate.
Flow Cytometry: 1:1,000 dilution from a previous lot of this antibody was used in FC.生物信息 免疫原品种 Synthetic peptide corresponding to residues on human MMP-9. 克隆 EP1254 宿主 Rabbit 特异性 This antibody recognizes MMP-9. 同种型 IgG 品种反应性 RatHuman Species Reactivity Note Human and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - P14780
基因符号 - OTTHUMP00000031674
- CLG4B
- EC 3.4.24.35
- GELB
- MMP-9
纯化方法 Unpurified UniProt编号 - P14780
UniProt汇总 FUNCTION: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
CATALYTIC ACTIVITY: Cleavage of gelatin types I and V and collagen types IV and V.
COFACTOR: Binds 2 zinc ions per subunit.
Binds 3 calcium ions per subunit.
ENZYME REGULATION: Inhibited by histatin-3 1/24 (histatin-5).
SUBUNIT STRUCTURE: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.
SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix Probable.
TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes.
INDUCTION: Activated by 4-aminophenylmercuric acetate and phorbol ester.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.
INVOLVEMENT IN DISEASE: Defects in MMP9 may be a cause of susceptibility to lumbar disk herniation (LDH) [MIM:603932]. LDH is the predominant cause of low-back pain and unilateral leg pain.
MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.
SEQUENCE SIMILARITIES: Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.产品使用声明 质量保证 Evaluated by Western Blot on HeLa cell lysate.
Western Blot Analysis: 1:5,000-20,000 dilution of this antibody was used to detect MMP-9 in U937, HL-60, and HT-1080 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.包装信息 数量 100 µL
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