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检测方法 Colorimetric 描述 概述 Detects and quantifies the levels of insulin receptor (IR) β-subunit independent of its phosphorylation status. The IR is a cell surface receptor that belongs to the superfamily of the growth factor receptor tyrosine kinases and regulates multiple signaling pathways. Although this kit is designed for use with human cell lines, it cross-reacts with mouse and rat cells. 产品目录编号 CBA039 品牌系列 Calbiochem®
参考 参考 Bevan, P. 2001. J. Cell. Sci. 114, 1429.
Skorey, K.I., et al. 2001. Anal. Biochem. 291, 269.
Ottensmeyer, F.P. et al. 2000. Biochemistry 39, 12103.
Playford, M.P., et al. 2000. Proc. Nat'l. Acad. Sci. USA 97, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest. 103, 931.
Wei, L., et al. 1995. J. Biol. Chem. 270, 8122.
Ebina, Y., et al. 1985. Cell 40, 747.
Ullrich, A., et al. 1985. Nature 313, 756.产品信息 检测方法 Colorimetric 形式 96 Tests 格式 96-well plate 试剂盒包含 IR (β-subunit) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. 正控制 IR (β-subunit) 生物信息 化验范围 0.94-60 ng/ml 化验时间 4 h 样品类型 Cells 品种 human, mouse, rat 理化信息 灵敏度 < 0.5 ng/ml 产品使用声明 预定用途 The Calbiochem® Insulin Receptor (β-subunit) ELISA Kit is designed to detect and quantify levels of the insulin receptor β-subunit independent of its phosphorylation status. Both natural (heterotetrameric) and recombinant IR react in this assay. This ELISA is not cross-reactive with IGF-1R. Although this ELISA kit is developed using cells containing human insulin receptor, mouse and rat insulin receptor will cross react. This assay is intended for detection of IR from lysates of cells. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
存储条件 Upon arrival store the entire contentsof the kit at 4°C. 无需冷冻 No
补充信息 试剂盒包含 IR (β-subunit) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol.
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