merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB3194,Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH

  • merck millipore,默克密理博,MAB3194,Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH

    产品名称：Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH
    产品型号：MAB3194
    Detect the Glutathione: N-ethylmaleimide adduct protein using this Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH validated for use in ELISA, Flow, ICC, IHC & WB.

    merck millipore,默克密理博,MAB3194,Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH

  • 产品介绍
  • merck millipore,默克密理博,MAB3194,Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    MaWB, ELISA, FC, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB3194
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH
    产品信息
    格式 Purified
    演示 Liquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
    应用
    应用 Detect the Glutathione: N-ethylmaleimide adduct protein using this Anti-Glutathione: N-ethylmaleimide adduct, clone 8.1GSH validated for use in ELISA, Flow, ICC, IHC & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Flow Cytometry
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunohistochemistry: 1:100 (paraformaldehyde fixation)

    Immunocytochemistry: 1:100 - 1:500 for assessment of intracellular GSH distribution.

    ELISA: 1:100,000 - 1:200,000 to quantify GSH at >2 mM in biological specimens.

    Flow cytometry: 1:100 - 1:1,000

    Cells were fixed with cold 1% PFA for 5 min and vortexed into methanol (-20°C) containing 5mM NEM (10 min on dry ice) or 10 μM NEM (N-ethyl maleimide) for 30 min at RT.

    Optimal working dilutions must be determined by end user.

    Positive Control: Peripheral blood mononuclear cells (PBMC) treated with NEM
    生物信息
    免疫原品种 GS-NEM conjugated to KLH
    克隆 8.1GSH
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Reactive with thiol-modified glutatione. Does not react with oxidized glutathione (GSSG) or glutathione (GSH) in mixed disulfide linkage with proteins. MAB3194 is specific to the glutathione:N-ethylmaleimide adduct; thus it is useful for quantification of reduced glutathione (GSH) after reaction with N-ethylmaleimide. For use in ELISA or flow cytometry, sample must first be reacted with NEM.
    同种型 IgG1
    品种反应性 Mammals
    抗体类型 Monoclonal Antibody
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2-8°C in undiluted.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB3194,Anti-Glutathione: N-ethylmaleimide adduct Antibody, clone 8.1GSH

上一件merck millipore产品：merck millipore,默克密理博,ECM430,α Integrin Blocking and IHC Kit
下一件merck millipore产品：merck millipore,默克密理博,MABD99,Anti-Nkx2.2 Antibody, clone 1C11


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