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品种反应性 主要应用 宿主 格式 抗体类型 H, R, M IHC, WB Gp Serum Polyclonal Antibody 描述 产品目录编号 AB2256 描述 Anti-GABA B Receptor R1 Antibody Alternate Names - seven transmembrane helix receptor
- GABA-B receptor
- GABA-B receptor 1
- GABAB, subunit 1c
- gamma-aminobutyric acid (GABA) B receptor 1
- gamma-aminobutyric acid B receptor, 1
- gamma-aminobutyric acid B receptor
- 1
背景信息 Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants. 产品信息 格式 Serum 控制 - Rat brain lysates
演示 GST-Depleted Sera with 0.05% NaN3. 应用 应用 Detect GABA B Receptor R1 using this Anti-GABA B Receptor R1 Antibody validated for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry: Working dilution of 1:3,000 in free floating mouse brain tissue. 生物信息 免疫原品种 Recombinant peptide (aa 972-991: PSEPPDRLSCDGSRVHLLYK) 表位 C-Terminus
interaction w/ ATF4宿主 Guinea Pig 特异性 Cat. # AB2256 recognizes GABA B Receptor R1 (GABA B R1) 品种反应性 HumanRatMouse Species Reactivity Note Proven to react with Rat, Mouse and Human. Reactivity with other species has not been determined. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_112290.2
Entrez基因汇总 metabotropic G-protein-coupled receptor for gamma-aminobutyric acid (GABA) [RGD] 基因符号 - hGB1a
- FLJ92613
- GABA-B-R1
- GABAB(1e)
- GABABR1
- GABBR1-3
- GPRC3A
- Gb1
UniProt编号 - Q9Z0U4
UniProt汇总 Function: Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
Cofactor: Calcium. Required for high affinity binding to GABA.
Subunit structure: Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Interacts with the leucine zipper of the C-terminal bZIP domain of ATF4 via its C-terminal region. Interacts with JAKMIP1.
Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes.
Tissue specificity: Isoform 1A, isoform 1B and isoform 1C are expressed in testis, stomach, spinal cord and brain including cerebral cortical layers, pyramidal cell layers of the hippocampus, granular cell layers of the dentate gyrus, basal ganglia, cerebellum (predominantly in Purkinje cells followed by granular layer). Isoform 1B is also expressed in kidney and liver. Isoform 1D is expressed in forebrain, cerebellum, eye, kidney, and urinary bladder.
Domain: Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
Sequence similarities: Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
Contains 2 Sushi (CCP/SCR) domains.
产品使用声明 质量保证 Western Blotting: 1:1,000 dilution from a previous lot detected GABA B R1 on 10µg of rat brain lysate. 储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 50 µL
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