- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
Replacement Information Replacement Information 07-1483 is a recommended replacement for AB3016. 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ELISA, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 07-1483 Replaces AB3016 描述 Anti-IκBα Antibody 背景信息 IKK beta (IκB Kinase, IKKβ, I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. IκB Kinase (IKK) complex is a critical component in NFκB regulation. In a majority of unstimulated cells, the NFκB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IκB proteins. For NFκB to become activated, it must first disassociate from the inhibitor IκB. This occurs via the serine phosphorylation of IκB on Ser32 and Ser36. Post receptor stimulation, most notably by TNFα, NIK, a serine/threonine kinase, activates and phosphorylates IKKα and IKKβ. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates, which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1. IKK then binds to and phosphorylates IκBα on the two serine residues. This phosphorylation marks IκB for ubiquination and destruction by the proteosome. This frees the NFκB complex and allows it to translocate to the nucleus. 产品信息 格式 Serum 控制 - HEK293 cell lysate.
Control Peptide: Included with the antibody is 50 μg (1 mg/mL) of IκBα control peptide. The peptide will block the specific interaction of AB3016 with the IκBα subunit. Control peptide should be used at 1.0 μg per 1.0 μL of antiserum used in assay. Optimal concentrations must be determined by the end user.
演示 Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium phosphate with 0.15 M NaCl, pH 7.2 and 0.1% sodium azide. 应用 应用 Detect IκBα using this Anti-IκBα Antibody validated for use in WB, IH(P), ELISA & IP. 主要应用 - ELISA
- Western Blotting
应用说明 Immunohistochemisty (paraffin): Representative testing from a previous lot.
Anti-IкBα staining on invasive ductal carcinoma tissue (Breast Cancer) was pretreated with citrate buffer, pH 6.0. A 1:1,000 diluted was used using IHC-Select® detection with HRP-DAB.
ELISA: Recommended
Immunoprecipitation: Recommended
Optimal dilutions must be determined by the end user.生物信息 免疫原品种 IκBα peptide corresponding to a region near the C-terminus of the human protein, conjugated to KLH. 表位 C-terminus 宿主 Rabbit 特异性 Recognizes IκBα, C-terminus. May react non-specifically with other proteins. Control peptide provided will compete only with the specific reaction of antiserum with IκBα. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse and Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_020529.1
Entrez基因汇总 NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, orIKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM].
基因符号 - MAD-3
- NFKBIA
- IKBA
- MAD3
- IkappaBalpha
- I-kappa-B-alpha
- NFKBI
- IkB-alpha
UniProt编号 - P25963
UniProt汇总 FUNCTION: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
SUBUNIT: Interacts with RELA; the interaction requires the nuclear import signal. Interacts with NKIRAS1 and NKIRAS2. Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with HBV protein X. Interacts with RWDD3; the interaction enhances sumoylation.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export (By similarity).
INDUCTION: Induced in adherent monocytes.
PTM: Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity.
PTM: Ubiquitinated; subsequent to stimulus-dependent phosphorylation on serines.
PTM: Sumoylated; sumoylation requires the presence of the nuclear import signal.
DISEASE: Defects in NFKBIA are a cause of autosomal dominant anhidrotic ectodermal dysplasia with immunodeficiency (AD-EDA-ID) [MIM:164008]. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia.
SIMILARITY: Belongs to the NF-kappa-B inhibitor family.
SIMILARITY: Contains 5 ANK repeats.
产品使用声明 质量保证 Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500 to 1:2,000 dilution of this lot detected IkBalpha on 10 μg of HEK293 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Both antibody and control are stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL - HEK293 cell lysate.
上一件merck millipore产品：merck millipore,默克密理博,AB10553,Anti-Prohormone Convertase PC1/PC3 Antibody
下一件merck millipore产品：merck millipore,默克密理博,ABN241,Anti-GluR1 Antibody
相关产品
merck millipore,默克密理博,495624,InSolution™ Olomoucine - Calbiochem
merck millipore,默克密理博,MAB3750,Anti-LEF-1 Antibody, all isoforms, clone 1C3.1D10
merck millipore,默克密理博,S4710-K,Biotin-XX-OBEA-dCTP (15 µmol)
merck millipore,默克密理博,AB5717-200UL,Anti-Kv3.3 Antibody
merck millipore,默克密理博,AB10553,Anti-Prohormone Convertase PC1/PC3 Antibody
%5BABN241_IHC(P)-ALL%5D.jpg "merck millipore,默克密理博,ABN241,Anti-GluR1 Antibody")
merck millipore,默克密理博,ABN241,Anti-GluR1 Antibody
merck millipore,默克密理博,NS170,Phosphorylated Neurofilament (pNF-H) Sandwich ELISA Kit
merck millipore,默克密理博,ABS609,Anti-ASNA1 Antibody
merck millipore,默克密理博,MAB10206,ANTI-EIAV Core Antigen (p26) Antibody, clone EIAV 12E8.1,MAB10206
merck millipore,默克密理博,CBA039,Insulin Receptor (β-subunit) ELISA Kit