- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,APT400,CaspaTag Pan-Caspase In Situ Assay Kit, Fluorescein
产品名称：CaspaTag Pan-Caspase In Situ Assay Kit, Fluorescein
产品型号：APT400
The In Situ FLICA Pan-Caspase Detection Kit for flow cytometry is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis.
merck millipore,默克密理博,APT400,CaspaTag Pan-Caspase In Situ Assay Kit, Fluorescein
- 产品介绍
重要规格表
品种反应性 主要应用 检测方法 Ma ACT, FC Fluorescent 描述 产品目录编号 APT400 品牌系列 Chemicon®
商名 - Chemicon
- CaspaTag
描述 CaspaTag Pan-Caspase In Situ Assay Kit, Fluorescein 概述 Apoptosis is an evolutionarily conserved form of cell suicide, which follows a specialized cellular process. The central component of this process is a cascade of proteolytic enzymes called caspases. These enzymes participate in a series of reactions that are triggered in response to pro-apoptotic signals and result in the cleavage of protein substrates, causing the disassembly of the cell (Slee et al. 1999).
Caspases have been identified in organisms ranging from C. elegans to humans. The mammalian caspases play distinct roles in apoptosis and inflammation. In apoptosis, caspases are responsible for proteolytic cleavages that lead to cell disassembly (effector caspases), and are involved in upstream regulatory events (initiator caspases). An active caspase consists of two large and two small subunits that form two heterodimers which associate in a tetramer (Walker et al. 1994; Wilson et al. 1994; Rotonda et al. 1996). In common with other proteases, caspases are synthesized as precursors that undergo proteolytic maturation, either autocatalytically or in a cascade by enzymes with similar specificity (Kumar 1999).
Caspase enzymes specifically recognize a 4 or 5 amino acid sequence on the target substrate which necessarily includes an aspartic acid residue. This residue is the target for cleavage, which occurs at the carbonyl end of the aspartic acid residue (Thornberry et al. 1997). Caspases can be detected via immunoprecipitation, immunoblotting techniques using caspase specific antibodies, or by employing fluorochrome substrates which become fluorescent upon cleavage by the caspase.
Test Principle:
CHEMICON's In Situ Caspase Detection Kits use a novel approach to detect active caspases. The methodology is based on Fluorochrome Inhibitors of Caspases (FLICA). The inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the inhibitor binds covalently to the active caspase (Ekert et al. 1999). This kit uses a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of caspase (FAM-VAD-FMK), which produces a green fluorescence. When added to a population of cells, the FAM-VAD-FMK probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the active caspase heterodimer, thereby inhibiting further enzymatic activity. The bound labeled reagent is retained within the cell, while any unbound reagent will diffuse out of the cell and is washed away. The green fluorescent signal is a direct measure of the amount of active caspase present in the cell at the time the reagent was added. Cells that contain the bound labeled reagent can be analyzed by 96-well plate-based fluorometry, fluorescence microscopy, or flow cytometry.
Application:
The CHEMICON In Situ FLICA Pan-Caspase Detection Kit is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis. The kit is for research use only. Not for use in diagnostic or therapeutic procedures.产品信息 部件 - Hoechst Stain: 1 mL at 200 μg/mL, ready-to-use
- FLICA Reagent (FAM-VAD-FMK): Four lyophilized vials
- 10X Wash Buffer: 60 mL
- Fixative (10% formaldehyde): 6 mL
- Propidium Iodide: 1 mL at 250 μg/mL, ready-to-use
检测方法 Fluorescent 应用 应用 The In Situ FLICA Pan-Caspase Detection Kit for flow cytometry is a fluorescent-based assay for detection of active caspases in cells undergoing apoptosis. 主要应用 - Activity Assay
- Flow Cytometry
生物信息 品种反应性 Mammals Entrez基因编号 - NM_033295.2
- NM_001223.3
- NM_033292.2
- NM_033293.2
- NM_033294.2
Entrez基因汇总 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with apoptosis defects seen in type II autoimmune lymphoproliferative syndrome. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. 基因符号 - Caspase-1 subunit p10].
- CASP1
- IL1BCE
- P45
- IL-1BC
- IL1BC
- p45
- IL1B-convertase
- CASP-1
- ICE
- EC 3.4.22.36 [Contains: Caspase-1 subunit p20
UniProt编号 - Q92851
- P29466
- P42575
- P42574
- P51878
- P55212
- P55211
- Q14790
UniProt汇总 FUNCTION: SwissProt: Q92851 # Isoform C is proteolytically inactive.
SIZE: 521 amino acids; 58951 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit (By similarity). Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10.
TISSUE SPECIFICITY: Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
PTM: Cleavage by granzyme B and autocatalytic activity generate the two active subunits. & Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE: SwissProt: Q92851 # Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects. & Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in CASP10 are a cause of gastric cancers [MIM:137215].
SIMILARITY: SwissProt: Q92851 ## Belongs to the peptidase C14 family. & Contains 2 DED (death effector) domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 · Store unopened kit materials at 2-8°C up to their expiration date.
· Reconstituted FLICA Reagent (150X) should be frozen at -20ºC for up to 6 months, and may be thawed twice during this time. Aliquot into separate amber tubes if desired. Protect from light at all times.
· Store diluted (1X) wash buffer up to -20ºC for 2 weeks.包装信息 数量 100 assays
上一件merck millipore产品：merck millipore,默克密理博,ABC379,Anti-ACVRL1 Antibody
下一件merck millipore产品：merck millipore,默克密理博,GF441,HumanKine TGF beta 3, Human Recombinant Xeno-Free
相关产品
merck millipore,默克密理博,05-1540,Anti-Gemin2 Antibody, clone 2E17
merck millipore,默克密理博,69746,pET-23b(+) DNA - Novagen
merck millipore,默克密理博,PHCC20060,Scepter 2.0 Handheld Automated Cell Counter, w/ 50pk of 60µM Sensors
merck millipore,默克密理博,567737,S1P4 Receptor Antagonist, CYM50358 - Calbiochem
merck millipore,默克密理博,ABC379,Anti-ACVRL1 Antibody
merck millipore,默克密理博,GF441,HumanKine TGF beta 3, Human Recombinant Xeno-Free
merck millipore,默克密理博,FCMAB218F,Milli-Mark Anti-CD19-FITC Antibody, clone 6D5
merck millipore,默克密理博,AB5032,Anti-Neural Cell Adhesion Molecule Antibody
merck millipore,默克密理博,MABF117,Anti-Myeloid differentiation-2 (MD-2) Antibody, clone IIC1
merck millipore,默克密理博,AB15554-50UL,Anti-AT2 Receptor Antibody