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merck millipore,默克密理博,APT142,MitoLight Mitochondrial Apoptosis Detection Kit
产品名称：MitoLight Mitochondrial Apoptosis Detection Kit
产品型号：APT142
The MitoLight Apoptosis Detection Kit for flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker.
merck millipore,默克密理博,APT142,MitoLight Mitochondrial Apoptosis Detection Kit
- 产品介绍
重要规格表
主要应用 检测方法 FC Fluorescent 描述 产品目录编号 APT142 品牌系列 Chemicon®
商名 - Chemicon
- MitoLight
描述 MitoLight® Mitochondrial Apoptosis Detection Kit 概述 Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.
MitoLight™ partitions differently in healthy cells than in apoptotic cells. Therefore, it has been possible to use a fluorescence ratioing technique to study mitochondrial membrane potentials. In healthy cells, the dye accumulates and aggregates in the mitochondria, giving off a bright red fluorescence (λem = 585-590 nm). In apoptotic cells with altered mitochondrial membrane potential, the dye in its monomeric form stays in the cytoplasm, fluorescing green (λem = 527-530 nm), providing a ready discrimination between apoptotic and nonapoptotic cells. The fluorescence can be observed by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530) and PI channel for red aggregates (Ex/Em = 488/585).
For research use only; Not for use in diagnostic procedures产品信息 部件 - 10X Incubation Buffer (Part No. 71614): 20 mL.
- MitoLight (Part No. 71612): 25 μL in DMSO.
检测方法 Fluorescent 应用 应用 The MitoLight Apoptosis Detection Kit for flow cytometry utilizes a lipophilic cation, termed as MitoLight, as a mitochondrial activity marker. 主要应用 - Flow Cytometry
生物信息 品种反应性 Mammals 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store kit materials at -20°C up to their expiration date. After thawing, unused kit components can be refrozen. For best results, aliquot the MitoLight™ reagent into separate vials to avoid repeated freeze/thaw cycles.
PROTECT REAGENTS FROM LIGHT.包装信息 数量 25 assays
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