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merck millipore,默克密理博,ABE460,Anti-dimethyl Histone H3 (Arg2), symmetric Antibody
产品名称:Anti-dimethyl Histone H3 (Arg2), symmetric Antibody
产品型号:ABE460
This Anti-dimethyl Histone H3 (Arg2) antibody, symmetric is validated for use in western blotting, dot blot, IHC & ChIP for the detection of dimethyl Histone H3 (Arg2).
merck millipore,默克密理博,ABE460,Anti-dimethyl Histone H3 (Arg2), symmetric Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ChIP, WB, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 ABE460 描述 Anti-dimethyl Histone H3 (Arg2), symmetric Antibody Alternate Names - Histone H3/l
- H3R2me2s
- Histone H3/a
- Histone H3/b
- Histone H3/c
- Histone H3/d
- Histone H3/f
- Histone H3/h
- Histone H3/i
- Histone H3/j
- Histone H3/k
背景信息 Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. 产品信息 格式 Serum 演示 Rabbit polyclonal serum containing 0.05% sodium azide. 应用 应用 This Anti-dimethyl Histone H3 (Arg2) antibody, symmetric is validated for use in western blotting, dot blot, IHC & ChIP for the detection of dimethyl Histone H3 (Arg2). 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
- Immunohistochemistry
应用说明 Dot Blot (Specificity) Analysis: A 1:1,000 dilution from a representative lot detected Histone H3 (Arg2) in an Absurance™ Histone H3 Specificity Array (Cat. No. 16-667).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Histone H3 (Arg2) in drosophila chromosomes (Migliori, V., et al. (2012). Nat Struct Mol Biol. 19(2):136-144.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3 (Arg2) from P493-6 cell lysate (Migliori, V., et al. (2012). Nat Struct Mol Biol. 19(2):136-144.).生物信息 免疫原品种 Linear peptide corresponding to human Histone H3 symmetrically dimethylated at Arg2. 表位 Methylated Arg2 宿主 Rabbit 品种反应性 Human Species Reactivity Note Human. Predicted to react with Mouse and Rat based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003520
基因符号 - H3FJ
- HIST1H3A
- H3FA
- HIST1H3B
- H3FL
- HIST1H3C
- H3FC
- HIST1H3D
- H3FB
- HIST1H3E
- H3FD
- HIST1H3F
- H3FI
- HIST1H3G
- H3FH
- HIST1H3H
- H3FK
- HIST1H3I
- H3FF
- HIST1H3J
修改 - Methylation
纯化方法 Unpurified UniProt编号 - P68431
产品使用声明 质量保证 Evaluated by Western Blotting in untreated and nocodazole treated HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected dimethyl Histone H3 (Arg2) in 10 µg of nocodazole treated HeLa cell lysate and demonstrated a loss of signal in untreated HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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