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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5731 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Nanog Antibody, NT Alternate Names - Nanog homeobox
- Homeobox transcription factor Nanog
背景信息 NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).产品信息 格式 Affinity Purified 控制 - Whole cell lysate from mouse embryonic stem cells.
演示 Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide. 应用 应用 Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB. 主要应用 - Western Blotting
应用说明 Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from mouse Nanog. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Antibody recognizes Nanog. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse and rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_024865.2
基因符号 - hNanog
- FLJ12581
- FLJ40451
纯化方法 Affinity Purfied UniProt编号 - Q9H9S0
UniProt汇总 Function: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation By similarity.
Subunit structure: Interacts with SMAD1 and SALL4 By similarity.
Subcellular location: Nucleus. Ref.10
Tissue specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Ref.1 Ref.2 Ref.3 Ref.5 Ref.8
Developmental stage: Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. Ref.10 Ref.3 Ref.8 Ref.6 Ref.9
Miscellaneous: Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11); except NANOGP8 which is a retrogene.
Sequence similarities Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.产品使用声明 质量保证 Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 µg
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