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merck millipore,默克密理博,ABE187,Anti-YB-1 Antibody
产品名称：Anti-YB-1 Antibody
产品型号：ABE187
Use Anti-YB-1 Antibody (rabbit polyclonal antibody) validated in WB, ICC, IP to detect YB-1 also known as CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, Y-box transcript More>>
merck millipore,默克密理博,ABE187,Anti-YB-1 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE187 描述 Anti-YB-1 Antibody Alternate Names - YB-1
- Nuclease-sensitive element-binding protein 1
- CCAAT-binding transcription factor I subunit A
- CBF-A
- DNA-binding protein B
- DBPB
- Enhancer factor I subunit A
- EFI-A
- Y-box transcription factor
- Y-box-binding protein 1
背景信息 Y-box-binding protein 1 (YB-1) is a member of the highly conserved Y-box protein family. This multifunctional protein binds single stranded DNA and RNA and functions as a regulator of transcription, RNA metabolism, and protein synthesis.
YB-1 promotes the separation of DNA strands containing mismatches or modifications by cisplatin. In vitro studies have also shown that YB-1 has endonucleolytic activity resulting in the introduction of nicks or breaks into double-stranded DNA. YB1 may play a role in DNA repair.
YB1 binds to Y-box sequences present in multiple gene promoters to positively or negatively regulate transcription. YB1 activates genes associated with proliferation and cancer, such as cyclin A, cyclin B1, and matrix metalloproteinase-2 and represses genes associated with cell death, such as the FAS cell death-associated receptor and the p53 tumor suppressor gene.
In RNA, YB-1 binds to splice sites in pre-mRNA and plays a role in determining splice site selection. The interaction of this protein with RNA in the cytoplasm stabilizes mRNA and appears to contribute to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors.
产品信息 格式 Affinity Purified 控制 - Huvec cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-YB-1 Antibody (rabbit polyclonal antibody) validated in WB, ICC, IP to detect YB-1 also known as CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, Y-box transcription factor. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected YB-1 in HeLa cells.
Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory for protein immunoprecipitation (Fukada, T., et al. (2003). EMBO J. 22(3):479-493).生物信息 免疫原品种 Linear peptide corresponding to human YB-1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with Human, Mouse, and Rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004550
基因符号 - YB1
- YBX1
- NSEP1
纯化方法 Affinity purified UniProt编号 - P67809
UniProt汇总 FUNCTION: Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors By similarity. Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
SUBUNIT STRUCTURE: Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1 By similarity. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Interacts with IGF2BP1 and RBBP6. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, MBNL1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Interacts (via C-terminus) with APEX1 (via N-terminus); the interaction is increased with APEX1 acetylated at 'Lys-6' and 'Lys-7'.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
PTM: Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
In the absence of phosphorylation the protein is retained in the cytoplasm.
Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus By similarity.
SEQUENCE SIMILARITIES: Contains 1 CSD (cold-shock) domain.
SEQUENCE CAUTION: The sequence AAA35750.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAA59949.1 differs from that shown. Reason: Frameshift at several positions.
The sequence AAA61308.1 differs from that shown. Reason: Frameshift at position 314.产品使用声明 质量保证 Evaluated by Western Blot in Huvec cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected YB-1 on 10 µg of Huvec cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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