merck millipore,默克密理愽
  • merck millipore,默克密理博,AP342P-7ML,Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP

  • merck millipore,默克密理博,AP342P-7ML,Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP

    产品名称：Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP
    产品型号：AP342P-7ML
    This Goat anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP is validated for use in IH, IH(P) for the detection of Rabbit IgG.

    merck millipore,默克密理博,AP342P-7ML,Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP

  • 产品介绍
  • merck millipore,默克密理博,AP342P-7ML,Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP

    描述
    产品目录编号 AP342P-7ML
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP
    概述 Affinity purified goat anti rabbit IgG, polymeric HRP linked antibody.
    背景信息 CHEMICON's IHC Amplification reagent utilizes a novel controlled polymerization technology to prepare polymeric AP-linker antibody conjugates. The reagent is derived from small, multifunctional, polymerizable linkers that are used to activate a mixture of enzymes and linker antibodies, with polymerization under controlled conditions. Although the end product is a heterogeneous conjugate, with different molecular sizes, shapes and enzyme/antibody ratios, all results suggest that knowledge of the those parameters seems not to be critical with regard to performance. Compared to conventional biotin-streptavidin based three-step kits, the IHC Amplification reagent has the advantage of simpler and faster protocols, higher amplification power, biotin free and more consistent immunostaining outcomes on a variety of tissue types and antibodies (Shi et al. 1999).
    产品信息
    格式 HRP
    演示 Liquid.
    应用
    应用 This Goat anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP is validated for use in IH, IH(P) for the detection of Rabbit IgG.
    主要应用
    • Immunohistochemistry
    应用说明 PROTOCOL:

    Immunohistochemistry: (All procedures should be performed at room temperature)

    · Deparaffinization, rehydration

    · Block endogenous peroxidase with 3% H2O2 in dd water for 10 minutes.

    · Perform antigen retrieval or enzyme digestion if necessary.

    1. Block with blocking solution, 10 minutes. Blot off blocking solution. No need to wash.

    2. Incubate the rabbit primary antibody for 30-60 minutes, wash with PBS or TBS for 5 minutes, two times. (Incubation times with the primary antibody will need to be determined by the end user.)

    3. Incubate with the ready-to-use Poly-HRP-Anti-Rabbit IgG for 30 minutes, wash with PBS or TBS for 5 minutes, two times.

    4. Incubate with DAB or AEC solution for 5 minutes, wash with dd water.

    5. Counterstain with hematoxylin and mount.

    NOTES:

    1. The system is biotin free so that blocking endogenous biotin is not necessary.

    2. A pre-primary antibody blocking with 10% normal goat serum is recommended (CHEMICON catalog number S26-100ML).
    生物信息
    浓缩 ready to use
    宿主 Goat
    品种反应性 Rabbit
    抗体类型 Polyclonal Antibody
    纯化方法 Immunoaffinity purified
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain product at 2-8°C for up to 12 months.

    WARNING:

    Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
    包装信息
    数量 7 mL

    merck millipore,默克密理博,AP342P-7ML,Goat Anti-Rabbit IgG Antibody, Poly-HRP, Ampli-HRP

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