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品种反应性 主要应用 宿主 格式 抗体类型 H ChIP, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 ABE387 描述 Anti-ATF4 Antibody Alternate Names - TaxREB67
- Cyclic AMP-dependent transcription factor ATF-4
- cAMP-dependent transcription factor ATF-4
- Activating transcription factor 4
- Cyclic AMP-responsive element-binding protein 2
- CREB-2
- cAMP-responsive element-binding protein 2<br />DNA-binding protein TAXREB67
- Tax-responsive enhancer element-binding protein 67
背景信息 Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation. 产品信息 格式 Serum 控制 - HisOH treated HepG2 cell lysate
演示 Rabbit polyclonal serum with 0.05% sodium azide. 应用 应用 Anti-ATF4 is an antibody targeting the ATF4 protein, validated for use in WB & ChIP. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected ATF4 in HEPG2 cell lysates (Su, N. and Kilberg, M. S., et al. (2008). J Biol Chem. 283(50):351016-35117.; Fu, L., et al. (2011). J Biol Chem. 286(42):36724-36738.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected ATF4 in HEPG2 and MEF cell lysates (Shan, J., et al. (2012). J Biol Chem. 287(43):36393-36403.).生物信息 免疫原品种 Recombinant protein corresponding to human ATF4. 宿主 Rabbit 同种型 IgG 品种反应性 Human Species Reactivity Note Demonstrated to react with Human and Mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001666.2
基因符号 - TXREB
- ATF4
- CREB2
纯化方法 Unpurified UniProt编号 - P18848
产品使用声明 质量保证 Evaluated by Western Blot in untreated and HisOH treated HepG2 cell lysate.
Western Blot Analysis: A 1:2,000 dilution from a representative lot detected ATF4 in 10 µg of HisOH treated HepG2 cell lysate (HisOH was used to induce cellular stress mediated eIF2a phosphorylation, allowing for subsequent detection of ATF4. For enhanced performance, end-users may try higher dilutions of this antibody. For better results, 1% SDS was used during the secondary antibody incubation step.)使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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