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merck millipore,默克密理博,ABE33,Anti-phospho-Chk2 (Thr68) Antibody
产品名称：Anti-phospho-Chk2 (Thr68) Antibody
产品型号：ABE33
Use Anti-phospho-Chk2 (Thr68) Antibody (rabbit polyclonal antibody) validated in WB to detect phospho-Chk2 (Thr68) also known as CHK2 checkpoint homolog (S. pombe), checkpoint-like protein CHK2, prote More>>
merck millipore,默克密理博,ABE33,Anti-phospho-Chk2 (Thr68) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rhesus Macaque, H, Po, Chp WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE33 描述 Anti-phospho-Chk2 (Thr68) Antibody Alternate Names - serine/threonine-protein kinase CHK2
- CHK2 (checkpoint, S.pombe) homolog
- CHK2 checkpoint homolog (S. pombe)
- checkpoint-like protein CHK2
- protein kinase CHK2
背景信息 Chk2 is a protein kinase activated and phosphorylated in response to DNA damage, especially DNA double-strand breaks. As a cell cycle checkpoint regulator it acts to prevent DNA replication and inhibit mitotic entry prior to the conclusion of DNA replication. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene mutated in breast and ovarian cancer.
There are 12 known isoforms of Chk2. The majority of which are expressed solely in the cell nucleus. The exception is isoform 10 which is found throughout the cell. High expression has been observed in spleen, peripheral blood leukocytes, colon and testis. Lower expression is observed in other tissues. Mutations in CHEK2 gene coding for Chk2 are associated with Li-Fraumeni syndrome 2, and have been observed in some patients that contracted prostate cancer and osteosarcoma.产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal containing PBS with 0.05% sodium azide. 应用 应用 Use Anti-phospho-Chk2 (Thr68) Antibody (rabbit polyclonal antibody) validated in WB to detect phospho-Chk2 (Thr68) also known as CHK2 checkpoint homolog (S. pombe), checkpoint-like protein CHK2, protein kinase CHK2. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: 1 µg/ml from a representative lot detected Chk2 on 10 µg of HEK293 cell lysate. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Chk2 phosphorylated at Thr68. 表位 Thr68 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Chk2 phosphorylated at Thr68. 品种反应性 Rhesus MacaqueHumanPigChimpanzee Species Reactivity Note Demonstrated to react with human. Predicted to react with pig, chimpanzee, and rhesus monkey based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_009125
Entrez基因汇总 In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. 基因符号 - RAD53
- CHEK2
- CDS1
- CHK2
- Cds1
- HuCds1
- LFS2
- PP1425
纯化方法 Affinity Purfied UniProt编号 - O96017
UniProt汇总 FUNCTION:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR:Magnesium.
ENZYME REGULATION: Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation.
SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note: Isoform 10 is present throughout the cell.
Isoform 4: Nucleus.
Isoform 7: Nucleus.
Isoform 9: Nucleus.
Isoform 12: Nucleus.
TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
PTM: Phosphorylated by PLK4.
INVOLVEMENT IN DISEASE: Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807].
Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
Contains 1 FHA domain.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Chk2 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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