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品种反应性 主要应用 宿主 格式 抗体类型 H, M ChIP, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE180 描述 Anti-LSF Antibody Alternate Names - Transcription factor LSF
- Alpha-globin transcription factor CP2
- SAA3 enhancer factor
背景信息 Transcription factor LSF (late simian virus 40 factor) also known as transcription factor LSF, CP2, LBP-1c, and SEF is expressed in a variety of cell types including brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. LSF is essential for cell cycle progression at the G1/S transition after reentry into the cell cycle from G0. LSF plays an important role in DNA synthesis and cell survival. A major cellular target of LSF is the thymidylate synthase (TS) gene coding for the rate limiting enzyme in the production of dTTP. Disruption of LSF function results in apoptosis at S phase or to cell cycle arrest at the G1/S transition. 产品信息 格式 Affinity Purified 控制 - Mouse brain tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-LSF Antibody is a Rabbit Polyclonal Antibody for detection of LSF also known as Alpha-globin transcription factor CP2 & has been validated in WB. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Chromatin Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory in ChIP. See Repetny, K.J., et al. (2009). 生物信息 免疫原品种 Linear peptide corresponding to human LSF. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 HumanMouse Species Reactivity Note Demonstrated to react with Mouse. Predicted to react with Human based on immunogen design. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_005644
Entrez基因汇总 Summary: This gene encodes a transcription factor that binds the alpha-globin promoter and activates transcription of the alpha-globin gene. The encoded protein regulates erythroid gene expression, plays a role in the transcriptional switch of globin gene promoters, and it activates many other cellular and viral gene promoters. The gene product interacts with certain inflammatory response factors, and polymorphisms of this gene may be involved in the pathogenesis of Alzheimer's disease. [provided by RefSeq]. 基因符号 - SEF
- TFCP2
- LSF
纯化方法 Affinity purified UniProt编号 - Q12800
UniProt汇总 FUNCTION: Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 By similarity. Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter.
SUBUNIT STRUCTURE: Binds to DNA as a dimer, isoform 3 does not bind to DNA or affect the binding of isoform 1 to DNA. Interacts with UBP1 and PIAS1, and is probably part of a complex containing TFCP2, UBP1 and PIAS1 (by similarity). Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous. Expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung (at protein level).
DEVELOPMENTAL STAGE: Expressed in fetal erythroid tissue.
MISCELLANEOUS: Authors noted that a 10-fold molar excess of isoform 3 over isoform 1 inhibited DNA-binding.
SEQUENCE SIMILARITIES: Belongs to the grh/CP2 family. CP2 subfamily.产品使用声明 质量保证 Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected LSF on 10 µg of mouse brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,MABN43,Anti-Apolipoprotein E4 (ApoE4) Antibody, clone 4E4
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