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品种反应性 主要应用 宿主 格式 抗体类型 M, H ChIP, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 ABE1044 描述 Anti-HSF 1 Antibody Alternate Names - HSTF 1
- Heat shock factor protein 1
- HSF 1
- Heat shock transcription factor 1
背景信息 HSF1, also known as Heat shock factor protein 1 (HSF-1) or Heat shock transcription factor 1 (HSTF-1), and encoded by the gene HSF1/HSTF1, is a DNA binding transcription factor that binds heat shock promoter elements (HSE’s) and activates transcription. In higher eukaryotes, HSF1 is unable to bind to HSE regions unless the cells are heat shocked. HSF1 is normally a monomer that interacts with HSP90 and other chaperones in a large complex in the cytoplasm. Upon heat shock HSF1 is released from the complex, dimerizes, and is transported into the nucleus. HSF1 is localized to the cytoplasm during normal growth the protein localizes to the nucleus upon heat shock. HSF1 is part of the cellular stress response, and HSF1 is known to interact with many other stress- induced proteins including NCOA6, SYMPK, RALBP1, HSF2, CEBPB, HSPA1A, Heat shock protein 90kDa alpha and HSPA. 产品信息 格式 Serum 演示 Rabbit polyclonal serum containing 0.05% sodium azide. 应用 应用 Anti-HSF 1 antibody is an antibody against HSF 1 for use in western blotting, IP & ChIP. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HEK293 cell lysates transfected with wild type or mutant hRP1-HA (Fujimoto, M., et al. (2012). Mol Cell. 48(2):182-194.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from hHSF1 transfected MEF cell lysate (Fujimoto, M., et al. (2010). Mol Cell Biol. 21(1):106-116.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from wild type and HSF2-null primary MEF cell lysates (Shinkawa, T., et al. (2011). Mol Cell Biol. 22(19):3571-3583.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HeLa and MEF cell lysates which were either untreated or heat shock treated (Prof. A. Nakai, Yamaguchi University School of Medicine).生物信息 免疫原品种 Recombinant protein corresponding to mouse HSF 1. 宿主 Rabbit 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_032322
基因符号 - HSTF1
- HSF1
纯化方法 Unpurified UniProt编号 - P38532
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting: A 1:1,000 dilution of this antibody detected HSF 1 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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