- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,ABD66,Anti-Adipose Triglyceride Lipase (PNPLA2) Antibody
产品名称：Anti-Adipose Triglyceride Lipase (PNPLA2) Antibody
产品型号：ABD66
Detect Adipose Triglyceride Lipase (PNPLA2) using this Anti-Adipose Triglyceride Lipase (PNPLA2) Antibody validated for use in WB, IH(P).
merck millipore,默克密理博,ABD66,Anti-Adipose Triglyceride Lipase (PNPLA2) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 B, H, M, R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABD66 描述 Anti-Adipose Triglyceride Lipase (PNPLA2) Antibody Alternate Names - TTS2
- Patatin-like phospholipase domain-containing protein 2
- Adipose triglyceride lipase
- Calcium-independent phospholipase A2
- Desnutrin
- IPLA2-zeta
- Pigment epithelium-derived factor
- TTS2.2
- Transport-secretion protein 2
背景信息 Adipose Triglyceride Lipase, also known as PNPLA2, is a member of the PNPLA family of proteins. It specifically hydrolyses triglycerides and may be involved in the degradation of adiposomes. This protein is expressed in adipose tissue, heart, skeletal muscle, and portions of the gastrointestinal tract. Adipose Triglyceride Lipase has acylglycerol transacylase activity and may act coordinately with LIPE/HLS within the lipolytic cascade. Mutations in Adipose Triglyceride Lipase have been associated with neutral lipid storage disease with myopathy. 产品信息 格式 Affinity Purified 控制 - NIH/3T3 L1 adipocytes
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect Adipose Triglyceride Lipase (PNPLA2) using this Anti-Adipose Triglyceride Lipase (PNPLA2) Antibody validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Adipose Triglyceride Lipase in human colon, rat skeletal muscle, and rat retina tissues. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the lumenal domain of human Adipose Triglyceride Lipase. 表位 Lumenal domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Adipose Triglyceride Lipase at the lumenal domain. 品种反应性 BovineHumanMouseRat Species Reactivity Note Demonstrated to react with Human, Mouse, and Rat.
Predicted to react with Bovine based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_065109
Entrez基因汇总 This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]. 基因符号 - ATGL
- PNPLA2
纯化方法 Protein A purified UniProt编号 - Q96AD5
UniProt汇总 FUNCTION: Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
CATALYTIC ACTIVITY: Triacylglycerol + H2O = diacylglycerol + a carboxylate.
ENZYME REGULATION: Inhibited by BEL ((E)-6-(bromomethylene)-3-(1-naphthalenyl)-2H-tetrahydropyran-2-one), a suicide substrate inhibitor. No differences in enzymatic activity that uses (1,2-dilinoleoyl)-phosphatidylcholine as substrate was detected in the presence or absence of ATP. Activated by ABHD5 and SERPINF1.
PATHWAY: Glycerolipid metabolism; triacylglycerol degradation.
SUBUNIT STRUCTURE: Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity (By similarity). Interacts with SERPINF1; interacts at one site of interaction. Despite a colocalization in lipid droplets, it probably does not interact with PLIN (By similarity).
SUBCELLULAR LOCATION: Lipid droplet. Cell membrane; Single-pass type II membrane protein
TISSUE SPECIFICITY: Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
DEVELOPMENTAL STAGE: Induced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium.
POLYMORPHISM: Genetic variations in PNPLA2 may be associated with plasma free fatty acids, triglycerides levels, and fasting glucose concentrations.
INVOLVEMENT IN DISEASE: Note=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells.
SEQUENCE SIMILARITIES: Contains 1 patatin domain.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence:
Optimum pH is 7.5 with (1,2-dilinoleoyl)-phosphatidylcholine as substrate.
SEQUENCE CAUTION: The sequence AAP34448.1 differs from that shown. Reason: Frameshift at position 501.
The sequence CAC01131.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAC01132.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in NIH/3T3 L1 adipocytes.
Western Blot Analysis: 1 µg/mL of this antibody detected Adipose Triglyceride Lipase on 10 µg of NIH/3T3 L1 adipocytes.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,04-1571,Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) Antibody, clone 3E10
下一件merck millipore产品：merck millipore,默克密理博,MAB4155C3,Anti-BCRP1 Antibody, clone 5D3, Cy3 conjugate
相关产品
merck millipore,默克密理博,1000-0785,1.5 ml Microcentrifuge tubes - Bag of 500 (conical bottom)
merck millipore,默克密理博,05-1044,Anti-Sin1 Antibody, clone 1C7.2
merck millipore,默克密理博,MAB3329,Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/63.1
merck millipore,默克密理博,ABC54,Anti-CASP8-associated protein 2 Antibody
merck millipore,默克密理博,MAB4155C3,Anti-BCRP1 Antibody, clone 5D3, Cy3 conjugate
merck millipore,默克密理博,MABE369,Anti-Suv4–20h2 Antibody, clone 6B9
merck millipore,默克密理博,MAB3572,Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone V/10
merck millipore,默克密理博,204879,Complement C1s, Human, Activated, Two-Chain Form