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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1044 描述 Anti-Sin1 Antibody, clone 1C7.2 Alternate Names - stress-activated protein kinase-interacting 1
- MEKK2-interacting protein 1
- Mitogen-activated protein kinase 2-associated protein 1
- SAPK-interacting protein 1
- Stress-activated map kinase-interacting protein 1
- TORC2 subunit MAPKAP1
- mitogen-activated protein kinase associated protein 1
- ras inhibitor MGC2745
- stress-activated map kinase interacting protein 1
背景信息 Sin1 (stress-activated protein kinase (SAPK)-interacting protein 1, MAPKAP1) is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase along with Sin1, GL (mLST8), Protor1, Protor2, and Rictor. The complex that is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Sin1 is also known to directly interact without proteins that include Ras, SPAKs, and JNK.
Sin1 is known to have multiple alternative splicing isoforms. Full-length Sin1 is a 522aa protein (59kDa). Sin1 (Isoform 5) (36 kDa) has exon 6 spliced to an alternative exon 7a that results in the loss of both the RBD and PHL domains. Sin1Isoform 2) (55 kDa) lacks exon 7 and lacks the RBD. Sin1 (Isoform 3) (53 kDa) lacks exon 8 lacks the PHL domain. Sin1 (Isoform 4) (37 kDa) lacks exon 1.
产品信息 格式 Purified 演示 Purified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% NaN3. 应用 应用 Detect Sin1 using this Anti-Sin1 Antibody, clone 1C7.2 validated for use in WB, IP, IH(P) & IF. 主要应用 - Western Blotting
生物信息 免疫原品种 Full-length Human Sin1 GST-fusion protein. 克隆 1C7.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Detects Sin-1 and its isoforms. 同种型 IgG2aκ 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, and Rat. Other species have not been tested. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001006618
Entrez基因汇总 This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq] 基因符号 - SIN1
- JC310
- MGC2745
- MIP1
纯化方法 Protein G purfied UniProt编号 - Q9BPZ7
UniProt汇总 Function: Essential component of the TORC2 complex, which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Ref.2 Ref.9 Ref.12 Ref.13 Ref.14
Subunit structure: All isoforms except isoform 4 are able to be part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2. Ref.2 Ref.12 Ref.14
Subcellular location: Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus. Ref.12 Ref.14
Tissue specificity: Ubiquitously expressed, with highest levels in heart and skeletal muscle. Ref.2
Sequence similarities: Belongs to the SIN1 family.
产品使用声明 质量保证 Western Blot Analysis:
This lot detected Sin1 at 1:1,000 dilution in A431 cell lysate resolved via SDS-PAGE and transferred to PVDF.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,1000-0785,1.5 ml Microcentrifuge tubes - Bag of 500 (conical bottom)
下一件merck millipore产品：merck millipore,默克密理博,MAB3329,Anti-MMP-14 Antibody, catalytic domain, clone LEM-2/63.1
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