- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 B, H, R, M, Eq, Rhesus Macaque WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABD52 描述 Anti-BAF57 Antibody Alternate Names - BAF57
- SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
- BRG1-associated factor 57
背景信息 BAF57, also known as SMARCE1, is a core subunit of the SWI/SNF complex, which is required for transcriptional activation of genes normally repressed by chromatin. The SWI/SNF chromatin remodeling complex is critical for androgen receptor transcriptional and the BAF57 subunit facilitates direct interaction with the receptor. Studies suggest a blockade of the androgen receptor-BAF57 interaction may target agonisty-induced androgen receptor function. Data also suggest that the SWI/SNF complex is required for transcriptional activation by the estrogen receptor. ChIP assays demonstrated BAF57 is recruited to the estrogen-response promoter, pS2. 产品信息 格式 Affinity Purified 控制 - Jurkat cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-BAF57 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC(P) to detect BAF57 also known as BRG1-associated factor 57 or BAF57. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: 0.5 µg/mL from a representative lot detected BAF57 on 10 µg of L6 cell lysate.
Immunohistochemistry Analysis: 1:300 dilution from a representative lot detected BAF57 in anal squamous cell carcinoma and ductal carcinoma tissue.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human BAF57 at the HMG box region. 表位 HMG box region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes BAF57 at the HMG box region. 品种反应性 BovineHumanRatMouseHorseRhesus Macaque Species Reactivity Note Demonstrated to react with Human and Rat. Predicted to react with Mouse, Equine, Rhesus Macaque, and Bovine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003070
Entrez基因汇总 The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq]. 基因符号 - BAF57
- SMARCE1
纯化方法 Affinity purified UniProt编号 - Q969G3
UniProt汇总 FUNCTION: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
SUBUNIT STRUCTURE: Component of 6 multiprotein chromatin-remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B), and at least SMARCE1, ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1 (SNF5/INI1). Other subunits specific to each of the complexes may also be present. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Also binds to the SRC/p160 family of histone acetyltransferases (HATs) composed of NCOA1, NCOA2, and NCOA3. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with RCOR1/CoREST, NR3C1 and ZMIM2/ZIMP7. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin (By similarity).
SUBCELLULAR LOCATION: Nucleus
DOMAIN: The HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development (By similarity).
SEQUENCE SIMILARITIES: Contains 1 HMG box DNA-binding domain.产品使用声明 质量保证 Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected BAF57 on 10 µg of Jurkat cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,04-393,Anti-phospho-p70 S6 Kinase (Thr421/Ser424) Antibody, rabbit monoclonal
下一件merck millipore产品：merck millipore,默克密理博,69436,pET-11a DNA - Novagen
相关产品
merck millipore,默克密理博,12-334,Rabbit Anti-Chicken IgY, bridging Antibody
merck millipore,默克密理博,524633,Phosphatase Inhibitor Cocktail Set IV, Lyophilized - Calbiochem
merck millipore,默克密理博,DR1089,Anti-NKX2-5 Mouse mAb (1E4-G5)
merck millipore,默克密理博,MAB3740,Anti-Protein Tyrosine Phosphatase µ Antibody, clone BK2
merck millipore,默克密理博,04-393,Anti-phospho-p70 S6 Kinase (Thr421/Ser424) Antibody, rabbit monoclonal
merck millipore,默克密理博,69436,pET-11a DNA - Novagen
merck millipore,默克密理博,AG565,Polycystin-2 Control Peptide for AB9088
merck millipore,默克密理博,MAB5634,Anti-Spastin Antibody, clone Sp3G11/1
merck millipore,默克密理博,14-818,Met (D1246N) Protein, active, 10 µg
merck millipore,默克密理博,15-103,PI3 Kinase/Akt/GSK3 Pathway Explorer Minipack