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品种反应性 主要应用 宿主 格式 抗体类型 R WB, IHC Rb Culture Supernatant Monoclonal Antibody 描述 产品目录编号 AB9864 Replaces AB1516; 07-362 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-NMDAR1 Antibody, rabbit monoclonal Alternate Names - glutamate receptor, ionotropic, N-methyl D-aspartate 1
- glutamate [NMDA] receptor subunit zeta 1
- NMDA receptor 1
- N-methyl-D-aspartate receptor subunit NR1
- N-methyl-D-aspartate receptor channel, subunit zeta-1
- glutamate [NMDA] receptor subunit zeta 1
- glutamate receptor
- ionotropic
背景信息 N-methyl-D-aspartate Receptors (NMDAR) are one of three pharmacologically distinct subtypes of ionotropic receptors that mediate a majority of excitatory neurotransmission in the brain via the endogenous amino acid, L-glutamate. MDARs form heteromeric complexes usually composed of two components, the NR1 subunit and NR2 subunits. Both proteins are weakly expressed throughout the brain but show localization in the dendritic region of the mushroom bodies. The NMDAR channel is highly permeable to Ca2+ and Na+ and its opening requires the simultaneous binding of glutamate and postsynaptic membrane depolarization. The activated NMDAR channel allows calcium influx into the post-synaptic cell where calcium triggers a cascade of biochemical events that create synaptic changes. NMDAR have been studied extensively in Drosophila for its potential relationship to behavioral plasticity. A newly identified NMDAR complex of more than 80 different proteins has been identified. Genetic and pharmacological disruptions of components of the NMDAR complex produce learning impairments in rodents, further linking behavioral plasticity to synaptic plasticity. 产品信息 格式 Culture Supernatant 控制 - Brain tissue
演示 Rabbit monoclonal supernatant liquid containing 0.05% sodium azide. 应用 应用 This Anti-NMDAR1 Antibody is validated for use in IH, WB for the detection of NMDAR1. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry:
AB9864 can be used for using 4% paraformaldehyde or paraformaldehyde/glutaraldehyde fixed rat tissue. Suggested working dilution for light microscopy is 1:1,000-1:2,000.
Western blot:
1:1,000-1:2,000 using ECL on rat brain lysate.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide corresponding to the C-terminus of rat NMDA receptor subunit (Catalog number AG344). {amino acids 909-938 rat NMDAR1{LQNQKDTVLPRRAIERE EGQLQLCSRHRES} 表位 a.a. 909-938 克隆 1.17.2.6 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The original sequence used is selective for splice variants NR1-1a, NR1-1b, NR1-2a, NR1-2b using the nomenclature defined by Hollmann, M. et al. (1993). These appear to be the major splice variants expressed in rat brain (Sugihara H, et al., 1992). No cross-reaction with other glutamate receptor subunits. Western Blot analysis shows that this rabbit monoclonal antibody labels a single band at approximately 120 kD corresponding to NMDAR1 in rat brain lysate. 同种型 IgG 品种反应性 Rat Species Reactivity Note Reactivity in mouse is weak. On reduced Western blots a band matching that in rat is observed but tests in immunohistochemistry have been unsuccessful. It is expected that the antibody will also react with monkey, gerbil, rabbit, feline and fish based on the fact that another antibody made to the same peptide sequence reacts with those species. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000832.5
Entrez基因汇总 The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. 基因符号 - NMDAR1
- GRIN1
- NR1
- Glutamate
- NMDAR
- NMDA1
纯化方法 Unpurified UniProt编号 - Q05586
UniProt汇总 FUNCTION: SwissProt: Q05586 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity).
SIZE: 938 amino acids; 105373 Da
SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Note=Enriched in post-synaptic plasma membrane and post-synaptic densities (By similarity).
PTM: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity (By similarity).
SIMILARITY: SwissProt: Q05586 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family产品使用声明 质量保证 Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected NMDAR1 on 10 μg of rat brain lysates使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 50 µL
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