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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M IHC, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9712 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Sortilin Antibody Alternate Names - Neurotensin receptor 3
背景信息 Neurotensin receptor 3 or sortilin (the intracellular sorting protein 95 kDa) is a member of a family of receptors characterized by a single transmembrane domain, a short intracytoplasmic domain, and a long cysteine-rich luminal extracellular domain. This receptor has been found in the brain, spinal cord, muscle and other peripheral cell types, and in cancer cells. Sortilin exhibits sequence similarities with proteins involved in intracellular transport and trafficking. 产品信息 格式 Serum 演示 Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material. 应用 应用 Anti-Sortilin Antibody is an antibody against Sortilin for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western blot: 1:1,000-1:2,000 on extracts from NSC34 cells.
Immunohistochemistry: 1:500-1:1,000 on rat spinal cord.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Recombinant protein from the extracellular domain of human sortilin. 宿主 Rabbit 特异性 Sortilin (Neurotensin receptor 3). By Western blot the antibody recognizes a strong band at ~90 kDa in extracts of NSC34 cells. By immunohistochemistry the antibody intensely stains motor neurons within the rat spinal cord. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002959.4
Entrez基因汇总 This gene encodes a protein that is a multi-ligand type-1 receptor with similarity to the yeast carboxypeptidase Y sorting receptor Vps10 protein. The encoded protein, a trans-Golgi network (TGN) transmembrane protein, binds a number of unrelated ligands that participate in a wide range of cellular processes; however, it lacks the typical features of a signalling receptor. In the TGN, furin mediates the activation of the mature binding form. The encoded protein consists of a large luminal domain, a single transmembrane segment and short C-terminal cytoplasmic tail. The luminal domain contains a cysteine-rich region similar to two corresponding segments in the yeast Vps10p; the cytoplasmic tail is similar to the corresponding segment of the cation-independent mannose 6-phosphate receptor and the tail also interacts with the VHS domains of GGA (Golgi-associated, gamma-adaptin homologous, ARF-interacting) proteins. 基因符号 - NTR3
- SORT1
- NT3
- Gp95
纯化方法 Serum UniProt编号 - Q99523
UniProt汇总 FUNCTION: SwissProt: Q99523 # Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
SIZE: 831 amino acids; 92068 Da
SUBUNIT: Interacts with LPL and SLC2A4 (By similarity). Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP, GM2A and PSAP. Forms a complex with NGFR which binds specifically to the precursor forms of NGFB (proNGFB) and BDNF (proBDNF).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Note=Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.TISSUE SPECIFICITY: Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.
DOMAIN: SwissProt: Q99523 The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N- terminal propeptide and the extracellular domain may also inhibit premature ligand binding. & The extracellular domain may be shed following protease cleavage in some cell types.
PTM: The N-terminal propeptide is cleaved by furin and possibly other homologous proteases. & Contains 8 intrachain disulfide bonds. & N-glycosylated.
SIMILARITY: Contains 9 BNR repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added 1:1 for additional stability. 包装信息 数量 50 µL
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