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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Op, Ch IH(P) Rb Serum Polyclonal Antibody 描述 产品目录编号 AB962 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Substance P Antibody, pain 产品信息 格式 Serum 演示 Liquid antiserum. Contains 0.1% sodium azide. 应用 应用 Anti-Substance P Antibody, pain is an antibody against Substance P for use in IH(P). 主要应用 - Immunohistochemistry (Paraffin)
应用说明 TISSUE EVALUATION:
Formalin-fixed and paraffin-embedded sections (6 microns thick) of median eminence tissue were prepared from the domestic chicken and opossum. Cryostat sections of the above tissues can also be used in the staining procedure.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4).
2. The antiserum diluted 1:500 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these two steps excess reagent is washed from the section as described before.
4. The final step in the staining involved treating section with a mixture of 3,3'-diamino-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20 in (PBS) for 30 minutes at 37*C.
3. After washing off the excess antiserum with PBS, the section is treated with fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
COMMENTS:
The antiserum described here has been established to give a positive staining of the nerve fibers in the medium eminence of two species using the above protocol. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time, e.g. 24 hours.生物信息 免疫原品种 Synthetic substance P bound to keyhole limpet hemocyanin (KLH) with carbodiimide. 宿主 Rabbit 特异性 The antiserum has been evaluated for its ability to stain nerve fibers in the external median eminence of the chicken and opossum using both the immunoperoxidase and immunofluorescence procedures. 品种反应性 OpossumChicken 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_013998.1
- NM_003182.1
- NM_013996.1
- NM_013997.1
Entrez基因汇总 This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. 基因符号 - TAC2
- TAC1
- NKNA
- Hs.2563
- NK2
- PPT
- NKA
UniProt编号 - P20366
UniProt汇总 FUNCTION: SwissProt: P20366 # Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
SIZE: 129 amino acids; 15003 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P20366 ## Belongs to the tachykinin family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,566380,Normal Goat Serum, Lyophilized Solid
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