merck millipore,默克密理愽
  • merck millipore,默克密理博,MABF316,Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11

  • merck millipore,默克密理博,MABF316,Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11

    产品名称：Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11
    产品型号：MABF316
    Detect CD3ε using this armenian hamster monoclonal antibody, Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11 validated for use in Flow Cytometry, IHC, Activity Assay, IP & western blotting.

    merck millipore,默克密理博,MABF316,Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11

  • 产品介绍
  • merck millipore,默克密理博,MABF316,Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    MWB, FC, IHC, ACTAHmAPCMonoclonal Antibody
    描述
    产品目录编号 MABF316
    描述 Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11
    Alternate Names
    • CD3e
    • T-cell surface glycoprotein CD3 epsilon chain
    • T-cell surface antigen T3/Leu-4 epsilon chain
    背景信息 CD3, also known as T3, is a member of the Ig and the ITAM superfamilies. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon and zeta. CD3 is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The CD3 antigen is present in early thymocytes, and has been considered the best all-around T cell marker. The positive staining of this marker may represent a sign of early commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface. The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies. CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
    产品信息
    格式 APC
    演示 Purified armenian hamster conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.
    应用
    应用 Detect CD3ε using this armenian hamster monoclonal antibody, Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11 validated for use in Flow Cytometry, IHC, Activity Assay, IP & western blotting.
    主要应用
    • Western Blotting
    • Flow Cytometry
    • Immunohistochemistry
    • Activity Assay
    应用说明 Western Blotting Analysis: A representative lot detected human CD3ε in Western Blotting. (Salvadori S, et al. 1994.)

    Immunohistochemistry Analysis: A representative lot detected human CD3ε in Immunohistochemistry. (Podd BS, et al. 2006; Tilley SL, et al. 2007)

    Immunofluorescent Analysis: A representative lot detected human CD3ε in Immunofuorecence. (Payer E, et al. 1991)

    Immunoprecipitation Analysis: A representative lot detected human CD3ε in Immunoprecipitation. (Leo O, et al. 1987.)

    Activity Assay Analysis: A representative lot detected human CD3ε in Activity Assay. (Leo O, et al. 1987; Kruisbeek AM, et al. 1991; Vossen ACTM, et al. 1995; Terrazas LI, et al. 2005; Wang W, et al. 2007.)
    生物信息
    免疫原品种 H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
    克隆 145-2C11
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Armenian Hamster
    同种型 IgG
    品种反应性 Mouse
    抗体类型 Monoclonal Antibody
    基因符号
    • Cd3e
    纯化方法 Affinity
    UniProt编号
    • P22646
    产品使用声明
    质量保证 The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

    Flow Cytometry Analysis: 1 μg of this antibody detected CD3ε in one million C57BL/6 mouse splenocytes.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MABF316,Anti-CD3ε (mouse) Antibody, APC, clone 145-2C11

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