merck millipore,默克密理愽
  • merck millipore,默克密理博,AB9260,Anti-Ki-67 Antibody

  • merck millipore,默克密理博,AB9260,Anti-Ki-67 Antibody

    产品名称：Anti-Ki-67 Antibody
    产品型号：AB9260
    Detect Ki-67 with Anti-Ki-67 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IHC(P) & WB.

    merck millipore,默克密理博,AB9260,Anti-Ki-67 Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB9260,Anti-Ki-67 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MWBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB9260
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Ki-67 Antibody
    背景信息 Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
    产品信息
    格式 Affinity Purified
    控制
    • Positive Control: Breast carcinoma, lymph node tissue, normal tonsil.
    演示 Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4 containing BSA and sodium azide.
    应用
    应用 Detect Ki-67 with Anti-Ki-67 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IHC(P) & WB.
    主要应用
    • Western Blotting
    应用说明 Immunocytochemistry:
    Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining).

    Western Blot Analysis:
    A previous lot of this antibody was used in western blot.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Synthetic peptide from human Ki-67
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes Ki-67
    品种反应性 RatHumanMouse
    Species Reactivity Note Human and mouse. Expected to react with rat based on sequence homology.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_002417.3
    基因符号
    • Ki-67
    • MKI67
    • KIA
    纯化方法 Immunoaffinity purified
    UniProt编号
    • P46013
    UniProt汇总 FUNCTION: SwissProt: P46013 # Thought to be required for maintaining cell proliferation.
    SIZE: 3256 amino acids; 358694 Da
    SUBUNIT: Binds through the FHA domain to MKI67IP.
    SUBCELLULAR LOCATION: Nucleus. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
    DEVELOPMENTAL STAGE: Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
    SIMILARITY: SwissProt: P46013 ## Contains 1 FHA domain.
    产品使用声明
    质量保证 Immunohistochemistry(paraffin):
    Representative lot data.
    This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8ºC from date of receipt.
    包装信息
    数量 500 µL

    merck millipore,默克密理博,AB9260,Anti-Ki-67 Antibody

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下一件merck millipore产品：merck millipore,默克密理博,MABN605,Anti-Pan-PTPR Antibody,clone N165/43


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