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品种反应性 主要应用 宿主 格式 抗体类型 Ox, H, R, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB6048 描述 Anti-Dynactin subunit 1 Antibody Alternate Names - Dynactin subunit 1<br />150 kDa dynein-associated polypeptide<br />DAP-150<br />Short name=DP-150<br />p135<br />p150-glued
背景信息 Dynactin is a processivity factor for cytoplasmic dynein. Dynactin is also important in the positioning of the mitotic spindle. Dynactin is involved in the forward movement of the growth cone and is essential for activating the dynein motor complex, which is important in many other cell functions such as mitosis. Dynactin seems to hold the metaphasic centrosome in place to ensure the transition to anaphase. 产品信息 格式 Affinity Purified 控制 - Human testis lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect Dynactin subunit 1 using this Anti-Dynactin subunit 1 Antibody validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Dynactin subunit 1 in rat cerebellum, rat cortex, human cerebellum, and human cortex tissues. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the coiled coil region of human Dynactin subunit 1. 表位 Coiled Coil Region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Dynactin subunit 1 at the coiled coil region. 品种反应性 OxHumanRatMouse Species Reactivity Note Demonstrated to react with Human, Rat, and Mouse.
Predicted to react with Ox based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004073
Entrez基因汇总 This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq]. 基因符号 - DCTN1
纯化方法 Affinity Purified UniProt编号 - Q14203
UniProt汇总 FUNCTION: Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
SUBUNIT STRUCTURE: Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein. Interacts with the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Interacts with FBXL5. Interacts with ECM29. Interacts (via C-terminus) with SNX6.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton.
TISSUE SPECIFICTY: Brain.
PTM: Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome.
INVOLVEMENT IN DISEASE: Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally.
SEQUENCE SIMILARITIES: Belongs to the dynactin 150 kDa subunit family.
Contains 1 CAP-Gly domain.产品使用声明 质量保证 Evaluated by Western Blot in human testis lysate.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected Dynactin subunit 1 in 10 µg of human testis lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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