merck millipore,默克密理愽
  • merck millipore,默克密理博,AB5889,Anti-Capsaicin Receptor Antibody, NT

  • merck millipore,默克密理博,AB5889,Anti-Capsaicin Receptor Antibody, NT

    产品名称：Anti-Capsaicin Receptor Antibody, NT
    产品型号：AB5889
    This Anti-Capsaicin Receptor Antibody, N-terminus is validated for use in WB, IH for the detection of Capsaicin Receptor.

    merck millipore,默克密理博,AB5889,Anti-Capsaicin Receptor Antibody, NT

  • 产品介绍
  • merck millipore,默克密理博,AB5889,Anti-Capsaicin Receptor Antibody, NT

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Pm, M, RIHC, WBRbSerumPolyclonal Antibody
    描述
    产品目录编号 AB5889
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Capsaicin Receptor Antibody, NT
    Alternate Names
    • VR1
    • Vanilloid Receptor 1
    产品信息
    格式 Serum
    演示 Liquid. Contains 0.05% sodium azide.
    应用
    应用 This Anti-Capsaicin Receptor Antibody, N-terminus is validated for use in WB, IH for the detection of Capsaicin Receptor.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    应用说明 Immunoblotting: 1:1,000

    Immunohistochemistry: 1:1,000

    Optimal working dilutions must be determined by the end user.

    APPLICATION NOTES FOR AB5889

    IMMUNOHISTOCHEMISTRY

    Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

    Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5889. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

    IMMUNOHISTOCHEMISTRY

    VR1 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.

    WESTERN BLOT

    For details see Guo, A., et al., Eur J Neurosci (1999) 11:946-958.
    生物信息
    免疫原品种 Synthetic peptide corresponding to the N-terminus of rat VR1.
    表位 N-terminus
    宿主 Rabbit
    特异性 Capsaicin Receptor (VR1), N-terminal.

    SPECIES REACTIVITIES:

    Rat, mouse and primate. Other species have not been tested.
    品种反应性 PrimateMouseRat
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_080706.3
    • NM_018727.5
    • NM_080704.3
    • NM_080705.3
    Entrez基因汇总 Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
    基因符号
    • DKFZp434K0220
    • TRPV1
    • VR1
    • OTRPC1
    • TrpV1
    UniProt编号
    • Q8NER1
    UniProt汇总 FUNCTION: SwissProt: Q8NER1 # Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL.
    SIZE: 839 amino acids; 94938 Da
    SUBUNIT: Self-associates. Probably homotetramer. May also form a heteromeric channel with TRPV3. Interacts with calmodulin, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity).
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
    TISSUE SPECIFICITY: Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).DOMAIN:SwissProt: Q8NER1 The association domain (AD) is necessary for self- association (By similarity).
    PTM: Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity (By similarity).
    SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats.
    MISCELLANEOUS: Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB5889,Anti-Capsaicin Receptor Antibody, NT

上一件merck millipore产品：merck millipore,默克密理博,MABN674,Anti-PSIP1 Antibody, clone 6E4
下一件merck millipore产品：merck millipore,默克密理博,ABC422,Anti-PTGES3 Antibody


相关产品

merck millipore,默克密理博,70006,T7SelectDOWN Primer merck millipore,默克密理博,70006,T7SelectDOWN Primer

merck millipore,默克密理博,AB16532,Anti-Survivin Antibody, NT merck millipore,默克密理博,AB16532,Anti-Survivin Antibody, NT

merck millipore,默克密理博,09-448,Anti-phospho-IRS1 (Ser612) mouse/ (Ser616) human Antibody merck millipore,默克密理博,09-448,Anti-phospho-IRS1 (Ser612) mouse/ (Ser616) human Antibody

merck millipore,默克密理博,AB2267,Anti-LCN2 Antibody merck millipore,默克密理博,AB2267,Anti-LCN2 Antibody

merck millipore,默克密理博,MABN674,Anti-PSIP1 Antibody, clone 6E4 merck millipore,默克密理博,MABN674,Anti-PSIP1 Antibody, clone 6E4

merck millipore,默克密理博,ABC422,Anti-PTGES3 Antibody merck millipore,默克密理博,ABC422,Anti-PTGES3 Antibody

merck millipore,默克密理博,APT1000,ApopTag ISOL Dual Fluorescence Apoptosis Detection Kit (DNase Types I & II) merck millipore,默克密理博,APT1000,ApopTag ISOL Dual Fluorescence Apoptosis Detection Kit (DNase Types I & I...

merck millipore,默克密理博,04-409,Anti-PTEN rabbit monoclonal Antibody merck millipore,默克密理博,04-409,Anti-PTEN rabbit monoclonal Antibody

merck millipore,默克密理博,ABN76,Anti-Neurofilament H (200 kDa) Antibody merck millipore,默克密理博,ABN76,Anti-Neurofilament H (200 kDa) Antibody

merck millipore,默克密理博,AB707-K,Goat Anti-Human Alpha-1-Antitrypsin (1mL) merck millipore,默克密理博,AB707-K,Goat Anti-Human Alpha-1-Antitrypsin (1mL)


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部