merck millipore,默克密理博,04-409,Anti-PTEN rabbit monoclonal Antibody

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merck millipore,默克密理博,04-409,Anti-PTEN rabbit monoclonal Antibody
产品名称：Anti-PTEN rabbit monoclonal Antibody
产品型号：04-409
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PTEN Rabbit Antibody detects level of PTEN & ha More>>

merck millipore,默克密理博,04-409,Anti-PTEN rabbit monoclonal Antibody

产品介绍

merck millipore,默克密理博,04-409,Anti-PTEN rabbit monoclonal Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, FC, IHC	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-409
品牌系列	Upstate
商名	Upstate
描述	Anti-PTEN rabbit monoclonal Antibody
Alternate Names	phosphatase and tensin homolog (mutated in multiple advanced cancers 1). MMAC1 phosphatase and tensin homolog deleted on chromosome 10 Mutated in multiple advanced cancers 1 phosphatase and tensin homolog
背景信息	PTEN acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. It also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. PTEN antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. It dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. Recent research shows a role for PTEN in addiction.

产品信息
格式	Purified
控制	MCF7 cells
演示	Rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PTEN Rabbit Antibody detects level of PTEN & has been published & validated for use in FC, IF, IH, IH(P), IP & WB.
主要应用	Western Blotting Flow Cytometry Immunohistochemistry
应用说明	Immunoprecipitation: A 1:50 dilution of a previous lot of this antibody immunoprecipitated PTEN from MCF-7 cell lysate using protein G:agarose beads. Flow Cytometry: A 1:20 dilution of a previous lot of this antibody stained cells for intracellular staining for flow cytometry analysis. Immunohistochemistry: A 1:50 dilution of a previous lot of this antibody was used for detection of PTEN in immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma. Immunfluorescence Staining: 1:50 dilution of a previous lot of this antibody was used for Immunfluorescence staining of paraformaldehyde fixed cells.

生物信息
免疫原品种	KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PTEN.
表位	C-terminus
宿主	Rabbit
特异性	This antibody recognizes the C-terminal domain of PTEN.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Reacts with human, mouse and rat
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_000314.4
Entrez基因汇总	This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
基因符号	MGC11227 PTEN TEP1 PTEN1 MMAC1 MHAM BZS
纯化方法	Purified
UniProt编号	P60484
UniProt汇总	FUNCTION: SwissProt: P60484 # Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. COFACTOR: Magnesium. SIZE: 403 amino acids; 47166 Da SUBUNIT: Monomer. The unphosphorylated form interacts with the second PDZ domain of AIP1 and with DLG1 and MAST2 in vitro. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas. DOMAIN: SwissProt: P60484 The C2 domain binds phospholipid membranes in vitro in a Ca(2+)-independent manner; this binding is important for its tumor suppressor function. PTM: Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit and promote PDZ-binding. DISEASE: SwissProt: P60484 # Mutations of PTEN are found in a large number of cancers. & Defects in PTEN are a cause of Cowden disease (CD) [MIM:158350]; also known as Cowden syndrome (CS). CD is an autosomal dominant cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid and skin. The predominant phenotype for CD is multiple hamartoma syndrome, in many organ systems including the breast (70% of CD patients), thyroid (40-60%), skin, CNS (40%), gastrointestinal tract. Affected individuals are at an increased risk of both breast and thyroid cancers. Trichilemmomas (benign tumors of the hair follicle infundibulum), and mucocutaneous papillomatosis (99%) are hallmarks of CD. & Defects in PTEN are the cause of Lhermitte-Duclos disease (LDD) [MIM:158350]; also known as cerebelloparenchymal disorder VI. LDD is characterized by dysplastic gangliocytoma of the cerebellum which often results in cerebellar signs and seizures. LDD and CD seem to be the same entity, and are considered as hamartoma-neoplasia syndromes. & Defects in PTEN are a cause of Bannayan-Zonana syndrome (BZS) [MIM:153480]; also known as Ruvalcaba-Riley-Smith or Bannayan-Riley-Ruvalcaba syndrome (BRRS). In BZS there seems not to be an increased risk of malignancy. It has a partial clinical overlap with CD. BZS is characterized by the classic triad of macrocephaly, lipomatosis and pigmented macules of the gland penis. & Defects in PTEN are a cause of squamous cell carcinoma of the head and neck (HNSCC) [MIM:275355]. & Defects in PTEN are a cause of susceptibility to endometrial cancer [MIM:608089]. & Defects in PTEN are a cause of Proteus syndrome [MIM:176920]. Proteus syndrome is a hamartomatous disorder characterized by overgrowth of multiple tissues, connective tissue and epidermal naevi, and vascular malformations. These presentations are usually apparent at birth or soon after and continue to develop as the patient ages. It is named after the Greek god Proteus who, legend has it, could change his shape at will to avoid capture. Tumors, mostly benign but some malignant, have also been reported in Proteus syndrome, generally presenting by the age of 20 years and including papillary adenocarcinoma of the testis, meningioma, and cystadenoma of the ovaries. & Defects in PTEN are a cause of oligodendroglioma [MIM:137800]; also called oligodendroblastoma or familial glioma of brain. Oligodendroglioma is a usually benign neoplasm derived from and composed of oligodendrogliocytes in varying stages of differentiation. The majority are seen in adults in the white matter of the brain. & Defects in PTEN are a cause of VACTERL association with hydrocephalus [MIM:276950]; which includes also VATER association with hydrocephalus. VACTERL is an acronym for vertebral anomalies, anal atresia, congenital cardiac disease, tracheoesophageal fistula, renal anomalies, radial dysplasia, and other limb defects. & Defects in PTEN are involved in prostate cancer [MIM:176807]. & Defects in PTEN are a cause of macrocephaly/autism syndrome [MIM:605309]. Patients have autism spectrum disorders and macrocephaly, with head circumferences ranging from +2.5 to +8 SD for age and sex (average head circumference +4.0 SD). SIMILARITY: Contains 1 C2 tensin-type domain. & Contains 1 phosphatase tensin-type domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on MCF7. Western Blot Analysis: A 1:500 dilution of the lot detected endogenous PTEN in lysates from MCF7 cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.