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品种反应性 主要应用 宿主 格式 抗体类型 R, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5856-50UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-VAMP-2 Antibody Alternate Names - Synaptobrevin 2
- Vesicle-Associated Membrane Protein 2
产品信息 格式 Affinity Purified 控制 - CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min) 应用 应用 Anti-VAMP-2 Antibody detects level of VAMP-2 & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Western blot: 1:1,000 using ECL on rat brain membranes.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 GST fusion protein corresponding to a cytoplasmic, N-terminal part of rat or mouse VAMP-2 (Accession numbers Q64357, NP 036795). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes VAMP-2 (Vesicle-Associated Membrane Protein 2, Synaptobrevin 2). The epitope does not share homology with any other known proteins.
SPECIES REACTIVITIES: It is expected that the antibody will also react with human, Macaca multata and bovine due to sequence homology (25/26). Other species have not been tested.品种反应性 RatMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_014232.1
Entrez基因汇总 The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. 基因符号 - SYB2
- VAMP2
- VAMP-2
- FLJ11460
- Synaptobrevin-2
UniProt编号 - P63027
UniProt汇总 FUNCTION: SwissProt: P63027 # Involved in the targeting and/or fusion of transport vesicles to their target membrane.
SIZE: 116 amino acids; 12649 Da
SUBUNIT: Interacts with VAPA and VAPB.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Note=Neuronal synaptic vesicles.
TISSUE SPECIFICITY: Nervous system and skeletal muscle.
SIMILARITY: SwissProt: P63027 ## Belongs to the synaptobrevin family. & Contains 1 v-SNARE coiled-coil homology domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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