merck millipore,默克密理博,AB5829,Anti-HuC Antibody

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merck millipore,默克密理博,AB5829,Anti-HuC Antibody
产品名称：Anti-HuC Antibody
产品型号：AB5829
Anti-HuC Antibody is an antibody against HuC for use in WB & IC.

merck millipore,默克密理博,AB5829,Anti-HuC Antibody

产品介绍

merck millipore,默克密理博,AB5829,Anti-HuC Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	ICC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB5829
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-HuC Antibody

产品信息
格式	Affinity Purified
演示	Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	Anti-HuC Antibody is an antibody against HuC for use in WB & IC.
主要应用	Immunocytochemistry Western Blotting
应用说明	Western blot: 1:200-1:1,000 using ECL. Immunocytochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Synthetic peptide, amino acids 20-36 of HuC.
宿主	Rabbit
特异性	Recognizes HuC. The molecular weight of the protein is 38,862 Daltons.
品种反应性	MouseHuman
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_032281.2 NM_001420.3
Entrez基因汇总	A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
基因符号	DKFZp547J036 ELAVL3 PLE21 HUCL MGC20653 HUC HuC
UniProt编号	Q14576
UniProt汇总	FUNCTION: SwissProt: Q14576 # May be involved in neuronal differentiation and maintenance. SIZE: 367 amino acids; 39547 Da TISSUE SPECIFICITY: Brain specific. SIMILARITY: SwissProt: Q14576 ## Belongs to the RRM elav family. & Contains 3 RRM (RNA recognition motif) domains.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

储存和货运信息

存储条件

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.