merck millipore,默克密理博,AB3879,Anti-Acetyl Lysine Antibody

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merck millipore,默克密理博,AB3879,Anti-Acetyl Lysine Antibody
产品名称：Anti-Acetyl Lysine Antibody
产品型号：AB3879
Anti-Acetyl Lysine Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Acetyl Lysine & has been validated in ELISA, IF, IHC, IP & WB.

merck millipore,默克密理博,AB3879,Anti-Acetyl Lysine Antibody

产品介绍

merck millipore,默克密理博,AB3879,Anti-Acetyl Lysine Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
A	WB, ELISA, IHC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB3879
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Acetyl Lysine Antibody
背景信息	In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

产品信息
格式	Affinity Purified
控制	TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
演示	Purified rabbit polyclonal in buffer containing PBS with 50% glycerol.

应用
应用	Anti-Acetyl Lysine Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Acetyl Lysine & has been validated in ELISA, IF, IHC, IP & WB.
主要应用	Western Blotting ELISA Immunohistochemistry
应用说明	Immunoprecipitation: A previous lot was used at 10 µg/mg crude protein. ELISA: 0.25-1 μg/mL of a previous lot was used. Immunohistochemistry: 2.5 μg/mL of a previous lot was used. Immunofluorescence: 5 μg/mL of a previous lot was used. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Acetylated KLH.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes proteins acetylated on lysine residues. This antibody recognizes multiple acetylated proteins including acetylated histones, acetylated BSA, and acetylated MBP, and exhibits no reaction with non-acetylated proteins.
品种反应性	All
Species Reactivity Note	Reacts with all species.
抗体类型	Polyclonal Antibody
修改	Acetylation
纯化方法	Immunoaffinity purified

产品使用声明
质量保证	Routinely evaluated by Western Blot on Hela Acid Extract/Sodium Butyrate lysates. Western Blot Analysis: 1:500 dilution of this lot detected lysine acylated proteins on 10 μg of Hela Acid Extract/Sodium Butyrate lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

包装信息
数量	100 µg

merck millipore,默克密理博,AB3879,Anti-Acetyl Lysine Antibody

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