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merck millipore,默克密理博,EZHTNFA,Human TNF-α ELISA Kit, EZHTNFA
产品名称：Human TNF-α ELISA Kit, EZHTNFA
产品型号：EZHTNFA
The Human TNF-α ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody.
merck millipore,默克密理博,EZHTNFA,Human TNF-α ELISA Kit, EZHTNFA
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重要规格表
可用的分析物 品种反应性 主要应用 TNF-α H ELISA 描述 产品目录编号 EZHTNFA 描述 Human TNF-α ELISA Kit, EZHTNFA 概述 The Human TNF-α ELISA Kit is a Sandwich Enzyme- Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. This kit is specifically designed for the accurate quantification of human TNF-α from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents. 背景信息 Human TNF-α (Tumor Necrosis Factor α), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble protein form derives from the membrane form by way of proteolytic processing. The membrane form protein, but not the soluble form, is phosphorylated on serine residues. Dephosphoryla- tion of the membrane occurs by binding to soluble TNFRSF1A/ TNFR1. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-α plays a critical role in many physiological and pathological conditions, including neurodegenerative diseases and cancer. 产品信息 精度（%） - Inter-assay: <15 %
- Intra-assay: <10 %
应用 应用 The Human TNF-α ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. 主要应用 - ELISA
生物信息 特异性 No cross-reactivity was observed when this kit was used to analyze 14 human recombinant cytokines/chemokines at up to 50 ng/mL and rat and mouse TNF-α. 品种反应性 Human 可用的分析物 - TNF-α
准确度 - 104%
理化信息 灵敏度 - 3.5 pg/mL
稀释的线性 Contact Technical Service for this information 标准曲线范围 - 15.6-1000 pg/mL
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 96-well strip plate
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