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merck millipore,默克密理博,AB5668P,Anti-Neurite Outgrowth Inhibitor B Antibody
产品名称：Anti-Neurite Outgrowth Inhibitor B Antibody
产品型号：AB5668P
This Anti-Neurite Outgrowth Inhibitor B Antibody is validated for use in ELISA, WB for the detection of Neurite Outgrowth Inhibitor B.
merck millipore,默克密理博,AB5668P,Anti-Neurite Outgrowth Inhibitor B Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5668P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neurite Outgrowth Inhibitor B Antibody Alternate Names - Nogo-B
产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in PBS, containing 0.1% BSA and 0.05% sodium azide. 应用 应用 This Anti-Neurite Outgrowth Inhibitor B Antibody is validated for use in ELISA, WB for the detection of Neurite Outgrowth Inhibitor B. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1-10 μg/mL using ECL. Nogo-B has a molecular weight of approximately 37 kDa (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).
Immunohistochemistry: not tested. It is recommended that the antibody be tried at 2-20 μg/mL on paraformaldehyde fixed tissue.
ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 A 12 amino acid peptide sequence within the N-terminus and at the splice site of Nogo-A from the putative extracellular domain of rat Nogo-B (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes rat Neurite Outgrowth Inhibitor Protein B (Nogo-B). The immunogen sequence is present only in Nogo-B and absent in Nogo-C.
SPECIES REACTIVITIES: The immunogen peptide is 83% conserved in human Nogo-B/Foocen-m/foocen-m2. Reactivity with other species has not been confirmed.品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_207521.1
- NM_007008.2
- NM_020532.4
- NM_153828.2
- NM_207520.1
Entrez基因汇总 This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. 基因符号 - Nogo-B
- RTN4
- RTN4-B1
- Nbla10545
- RTN-X
- reticulon-4A
- Nogo-C
- NSP
- RTN-x
- ASY
- foocen
- NSP-CL
- NOGOC
- RTN4-A
- Reticulon-4
- Nbla00271
- KIAA0886
- NOGOA
- Reticulon-5
- Foocen
- NI220/250
- RTN4-C
- RTN4-B2
- SP1507
- NOGO-A
- NOGO
- NOGOB
UniProt编号 - Q9NQC3
UniProt汇总 FUNCTION: SwissProt: Q9NQC3 # Potent neurite growth inhibitor in vitro and plays a role both in the restriction of axonal regeneration after injury and in structural plasticity in the CNS. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
SIZE: 1192 amino acids; 129931 Da
SUBUNIT: Binds to RTN4R. Interacts with Bcl-xl and Bcl-2. Isoform 2 binds to NGBR and RTN3. Isoform 2 and isoform 3 interact with BACE1 and BACE2.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
TISSUE SPECIFICITY: Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis- specific.
DOMAIN: SwissProt: Q9NQC3 Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo- 66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor (By similarity).
SIMILARITY: Contains 1 reticulon domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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