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品种反应性 主要应用 宿主 格式 抗体类型 H, Gp IHC, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB13458 Replaces 04-1150 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MMP-9 Antibody Alternate Names - GELB
- 92 kDa Type IV Collagenase
- 92 kDa gelatinase
- Gelatinase B
背景信息 Matrix metalloproteinases (also named matrixin or MMPs) are a major group of enzymes that regulate cell-matrix composition by using zinc for their proteolytic activities. They are essential for various normal biological processes such as embryonic development, morphogenesis, reproduction tissue resorption, and remodeling. Metalloproteinases also play a role in pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and cancer. The metalloproteinase MMP-9 is associated with a variety of autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, systemic sclerosis, rheumatoid arthritis, multiple sclerosis, polymyositis and atherosclerosis. MMP-9 plays either a primary or secondary role in each one of these autoimmune diseases by its up or down-regulation. It is not expressed constantly but rather is induced or suppressed by many regulating molecules. This feature of MMP-9 along with its involvement in disease pathogenesis turns it into a target for therapy in the treatment of potentially many autoimmune diseases.
Like other proteolytic enzymes, MMP-9 is first synthesized as inactive proenzyme or zymogens. activation of proMMP-9 is mediated by plasminogen activator/plasmin (PA/plasmin) system. The regulation of MMP-9 activity is also controlled through TIMP-3.
MMP-9 expression is regulated by several cytokines and growth factors, including interleukins, interferons, EGF, NGF, bFGF, VEGF, PDGF, TNF-alpha, TGF-beta, the extracellular matrix metalloproteinase inducer EMMPRIN and also osteopontin. Many of these stimuli induce the expression and/or activation of c-fos and c-jun proto-oncogene products, which heterodimerize and bind activator protein-1 (AP-1) sites within of MMP9 gene promoters.
Normally MMP-9’s primary function is the degradation of the extracellular matrix. It can degrade decorin, elastin, fibrillin, laminin, gelatin (denatured collagen), and types IV, V, XI and XVI collagen and also activates growth factors like proTGF-beta and proTNF-alpha. Physiologically, MMP-9 in coordination with other MMPs, play a role in normal tissue remodeling events such as neurite gowth, embryonic development, angiogenesis, ovulation, mammary gland involution and wound healing. MMP-9 with other MMPs is also involved in osteoblastic bone formation and/or inhibits osteoclastic bone resorption.
产品信息 格式 Purified 控制 - POSITIVE CONTROL:
Conditioned, serum-free medium from TPA-treated human fibrosarcoma (HT-1080) cells. Placenta or breast carcinoma.
演示 Purified immunogobulin by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide. 应用 应用 Anti-MMP-9 Antibody detects level of MMP-9 & has been published & validated for use in WB, IH, IH(P). 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistology (formalin/paraffin): 10-20μg/ml for 30 min at RT. No special pretreatment is required for histochemical staining of formalin/paraffin tissues.
Western Blot: 2-4μg/mL for 2 hours at RT
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Amino acids 626-644 of human MMP-9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 AB13458 recognizes proteins of 92kDa and ~86kDa which are identified as pro (latent) and activated forms of matrix metalloproteinase 9 (MMP-9; also known as 92kDa collagenase IV or gelatinase B). CHEMICON AB13458 shows no cross-reaction with pro and active forms of other MMPs.
CELLULAR LOCALIZATION:Enzyme is localized within the cytoplasm.品种反应性 HumanGuinea Pig 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004994.2
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. 基因符号 - CLG4B
- MMP9
- MMP-9
- GELB
UniProt编号 - P14780
UniProt汇总 FUNCTION: SwissProt: P14780 # May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond.
COFACTOR: Binds 2 zinc ions per subunit. & Binds 3 calcium ions per subunit.
SIZE: 707 amino acids; 78427 Da
SUBUNIT: Exists as monomer, disulfide-linked homodimer, and as a heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form.
TISSUE SPECIFICITY: Produced by normal alveolar macrophages and granulocytes.
DOMAIN: SwissProt: P14780 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains.
MISCELLANEOUS: In the arthritis patient this enzyme might contribute to the pathogenesis of joint destruction and might constitute a useful marker of disease status.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Antibody is stable for 12 months after date of receipt when stored at 2-8ºC. 包装信息 数量 500 µL
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