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品种反应性 主要应用 宿主 格式 抗体类型 R WB, ICC, IHC Ch Purified Polyclonal Antibody 描述 产品目录编号 AB5543 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-MAP2 Antibody 产品信息 格式 Purified 控制 - Positive = cerebral cortex. Negative = liver or kidney.
演示 Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide. 应用 应用 Anti-MAP2 Antibody is an antibody against MAP2 for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blot: 1:5,000-1:10,000 using ECL. Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.
Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.
Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Purified bovine brain MAP2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Chicken 特异性 Microtubule Associated Protein 2 (MAP2). It is expected that the antibody will react with all mammals. 品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_031847.2
- NM_001039538.1
- NM_002374.3
- NM_031845.2
Entrez基因汇总 This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described. 基因符号 - MAP2A
- MAP2
- MAP2B
- MAP-2
- MAP2C
- DKFZp686I2148
纯化方法 Ammonium sulfate precipitation UniProt编号 - P11137
UniProt汇总 FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
SIZE: 1827 amino acids; 199539 Da
PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 50 µL
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