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品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB Rb Serum Polyclonal Antibody 描述 产品目录编号 07-313 品牌系列 Upstate
商名 - Upstate
描述 Anti-Cyclophilin A Antibody 背景信息 Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP).
Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506.
Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.产品信息 格式 Serum 控制 - Positive Control Included: Non-Stim A431 Cell Lysate (12-301).
Other controls: Rat lung and rabbit liver cells.
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
演示 Rabbit polyclonal antiserum IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. 应用 应用 Anti-Cyclophilin A Antibody is an antibody against Cyclophilin A for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Recombinant full length protein corresponding to human Cyclophilin A. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Cyclophilin A, MW ~18 kDa. Additional bands may be detected at higher concentrations of the antibody. 同种型 IgG 品种反应性 MouseHumanRat Species Reactivity Note Human, mouse and rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_066953
Entrez基因汇总 This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq]. 基因符号 - CYPA
- LOC654188
纯化方法 Unpurified UniProt编号 - P62937
UniProt汇总 FUNCTION: SwissProt: P62937 # PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
SIZE: 165 amino acids; 18012 Da
SUBUNIT: Interacts with HIV-1 Capsid protein.
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: SwissProt: P62937 ## Belongs to the cyclophilin-type PPIase family. PPIase A subfamily. & Contains 1 PPIase cyclophilin-type domain.产品使用声明 质量保证 Evaluated by western blot on RIPA lysates from non-stimulated A431 and rat lung cells.
Western Blot Analysis:
1:1000-1:10,000 dilution of this antibody detected Cyclophilin A in RIPA lysates from non-stimulated A431 cells. A previous lot detected Cyclophilin A in RIPA lysates from rat lung cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 200 µL
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