merck millipore,默克密理愽
  • merck millipore,默克密理博,AB3080,Anti-Green Fluorescent Protein Antibody

  • merck millipore,默克密理博,AB3080,Anti-Green Fluorescent Protein Antibody

    产品名称：Anti-Green Fluorescent Protein Antibody
    产品型号：AB3080
    This Anti-Green Fluorescent Protein Antibody is validated for use in ELISA, IC, IH, WB for the detection of Green Fluorescent Protein.

    merck millipore,默克密理博,AB3080,Anti-Green Fluorescent Protein Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB3080,Anti-Green Fluorescent Protein Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    VrtWB, ELISA, ICC, IHCRbPurifiedPolyclonal Antibody
    描述
    产品目录编号 AB3080
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Green Fluorescent Protein Antibody
    Alternate Names
    • eGFP
    • GFP
    背景信息 The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
    产品信息
    格式 Purified
    控制
    • RENCX cells
    • GFP fusion protein expressed in cells
    演示 Purified rabbit polyclonal IgG in 10 mM Tris buffer, pH 7.2 with 0.01% sodium azide.
    应用
    应用 This Anti-Green Fluorescent Protein Antibody is validated for use in ELISA, IC, IH, WB for the detection of Green Fluorescent Protein.
    主要应用
    • Western Blotting
    • ELISA
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Western Blot:
    A previous lot of this antibody was used in western blot.

    ELISA:
    A 1:2000-1:10,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay.

    Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore’s highly-purified secondary antibodies in indirect detection systems.

    Immunocytochemistry:

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Highly purified native GFP from Aequorea victoria.
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 AB3080 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080 GFP antibody has been purified by Protein A chromatography, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.
    品种反应性 Vertebrates
    抗体类型 Polyclonal Antibody
    基因符号
    • eGFP
    • GFP
    纯化方法 Protein A purfied
    UniProt编号
    • P42212
    产品使用声明
    质量保证 Routinely evaluated by immunocytochemistry on RENCX cells.

    Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot of anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20ºC from date of receipt.

    Handling Recommendations:
    Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,AB3080,Anti-Green Fluorescent Protein Antibody

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