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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R IHC Gp Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5519 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Prodynorphin Antibody 产品信息 格式 Affinity Purified 演示 Affinity purified Guinea pig antibody in PBS containing 0.05% sodium azide. 应用 应用 Anti-Prodynorphin Antibody is an antibody against Prodynorphin for use in IH. 主要应用 - Immunohistochemistry
应用说明 Immunohistochemistry: 1:500
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5519
IMMUNOHISTOCHEMISTRY
Perfusion: 1) Ca2+-free Tyrode's solution followed by 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5519. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.生物信息 免疫原品种 Synthetic peptide from rat ProDynorphin. 宿主 Guinea Pig 特异性 ProDynorphin, rat. 品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_024411.2
Entrez基因汇总 The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. 基因符号 - leu-enkephalin
- PDYN
- MGC26418
- preprodynorphin
- leumorphin
- beta-neoendorphin-dynorphin
- rimorphin
- prodynorphin
- PENKB
- Preprodynorphin
UniProt编号 - P01213
UniProt汇总 FUNCTION: SwissProt: P01213 # Leumorphin has a typical opiod activity and may have anti-apoptotic effect (By similarity).
SIZE: 254 amino acids; 28385 Da
SUBCELLULAR LOCATION: Secreted.
PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
SIMILARITY: SwissProt: P01213 ## Belongs to the opioid neuropeptide precursor family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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下一件merck millipore产品：merck millipore,默克密理博,14-817M,Met (M1268T) Protein, active, 250 µg
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